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10.1002/art.39606 http://scihub22266oqcxt.onion/10.1002/art.39606 C5398095!5398095!26815131     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Arthritis+Rheumatol 2016 ; 68 (7): 1758-68 Nephropedia Template TP {{tp|p=26815131|t=2016. Next Generation Sequencing Reveals Restriction and Clonotypic Expansion of Regulatory T Cells in Juvenile Idiopathic Arthritis |pdf=|usr=}}{{26815131}} gab.com Text Next Generation Sequencing Reveals Restriction and Clonotypic Expansion of Regulatory T Cells in Juvenile Idiopathic Arthritis JO: Arthritis Rheumatol http://www.kidney.de/pget.php?&tw=1&pmid=26815131 #FOAvip Objective: Regulatory T (Treg) cell mediated suppression of effector T (Teff) cells is impaired in juvenile idiopathic arthritis (JIA); however, the basis for this dysfunction is incompletely understood. Animal models of autoimmunity and immunodeficiency demonstrate that a diverse Treg repertoire is essential to maintain Treg cell function. We therefore employed next generation sequencing to investigate the Treg and Teff repertoires in JIA. Methods: Treg (CD4+CD25+CD127lo) and Teff (CD4+CD25?) cells were isolated from peripheral blood and synovial fluid obtained from JIA patients, controls, and children with Lyme arthritis. Treg cell function was measured in suppressive assays. The T cell receptor ? chain (TRB) was amplified by multiplex PCR, and amplicons were sequenced with the Illumina HiSeq platform. Data was analyzed using ImmunoSEQ?, International ImMunoGeneTics system, and the Immunoglobulin Analysis Tools. Results: Compared to controls, the JIA peripheral blood Treg repertoire was restricted and clonotypic expansions were found in both blood and synovial fluid Treg cells. Skewed usage and pairing of TRB variable and joining genes, including overuse of gene segments that have been associated with other autoimmune conditions, was observed. JIA patients shared a substantial portion of synovial fluid Treg clonotypes that were private to JIA and not identified in Lyme arthritis. Conclusions: Our data identified restriction and clonotypic expansions in the JIA Treg repertoire with sharing of Treg clonotypes across arthritis patients. These findings suggest that abnormalities in the Treg repertoire, possibly engendered by shared antigenic triggers, may contribute to disease pathogenesis in JIA. Twit Text FOAVip Next Generation Sequencing Reveals Restriction and Clonotypic Expansion of Regulatory T Cells in Juvenile Idiopathic Arthritis ????Arthritis Rheumatol http://www.kidney.de/pget.php?&tw=1&pmid=26815131 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26815131 #FOAvip English Wikipedia <ref>{{Cite pmid|26815131}}</ref> Next Generation Sequencing Reveals Restriction and Clonotypic Expansion of Regulatory T Cells in Juvenile Idiopathic Arthritis #MMPMID26815131 Henderson LA; Volpi S; Frugoni F; Janssen E; Kim S; Sundel RP; Dedeoglu F; Lo MS; Hazen MM; Son MB; Mathieu R; Zurakowski D; Yu N; Lebedeva T; Fuhlbrigge RC; Walter JE; Lee YN; Nigrovic PA; Notarangelo LD Arthritis Rheumatol 2016[Jul]; 68 (7): 1758-68 PMID26815131show ga Objective: Regulatory T (Treg) cell mediated suppression of effector T (Teff) cells is impaired in juvenile idiopathic arthritis (JIA); however, the basis for this dysfunction is incompletely understood. Animal models of autoimmunity and immunodeficiency demonstrate that a diverse Treg repertoire is essential to maintain Treg cell function. We therefore employed next generation sequencing to investigate the Treg and Teff repertoires in JIA. Methods: Treg (CD4+CD25+CD127lo) and Teff (CD4+CD25?) cells were isolated from peripheral blood and synovial fluid obtained from JIA patients, controls, and children with Lyme arthritis. Treg cell function was measured in suppressive assays. The T cell receptor ? chain (TRB) was amplified by multiplex PCR, and amplicons were sequenced with the Illumina HiSeq platform. Data was analyzed using ImmunoSEQ?, International ImMunoGeneTics system, and the Immunoglobulin Analysis Tools. Results: Compared to controls, the JIA peripheral blood Treg repertoire was restricted and clonotypic expansions were found in both blood and synovial fluid Treg cells. Skewed usage and pairing of TRB variable and joining genes, including overuse of gene segments that have been associated with other autoimmune conditions, was observed. JIA patients shared a substantial portion of synovial fluid Treg clonotypes that were private to JIA and not identified in Lyme arthritis. Conclusions: Our data identified restriction and clonotypic expansions in the JIA Treg repertoire with sharing of Treg clonotypes across arthritis patients. These findings suggest that abnormalities in the Treg repertoire, possibly engendered by shared antigenic triggers, may contribute to disease pathogenesis in JIA. äNext Generation Sequencing Reveals Restriction and Clonotypic Expansio(epmc).pdf DeepDyve Pubget Overpricing