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Label-free single-cell separation and imaging of cancer cells using an integrated
microfluidic system
#MMPMID28425472
Antfolk M
; Kim SH
; Koizumi S
; Fujii T
; Laurell T
Sci Rep
2017[Apr]; 7
(?): 46507
PMID28425472
show ga
The incidence of cancer is increasing worldwide and metastatic disease, through
the spread of circulating tumor cells (CTCs), is responsible for the majority of
the cancer deaths. Accurate monitoring of CTC levels in blood provides clinical
information supporting therapeutic decision making, and improved methods for CTC
enumeration are asked for. Microfluidics has been extensively used for this
purpose but most methods require several post-separation processing steps
including concentration of the sample before analysis. This induces a high risk
of sample loss of the collected rare cells. Here, an integrated system is
presented that efficiently eliminates this risk by integrating label-free
separation with single cell arraying of the target cell population, enabling
direct on-chip tumor cell identification and enumeration. Prostate cancer cells
(DU145) spiked into a sample with whole blood concentration of the peripheral
blood mononuclear cell (PBMC) fraction were efficiently separated and trapped at
a recovery of 76.2?±?5.9% of the cancer cells and a minute contamination of
0.12?±?0.04% PBMCs while simultaneously enabling a 20x volumetric concentration.
This constitutes a first step towards a fully integrated system for rapid
label-free separation and on-chip phenotypic characterization of circulating
tumor cells from peripheral venous blood in clinical practice.
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