Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Stem+Cells 2017 ; 35 (4): 952-66 Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Quaking Is a Key Regulator of Endothelial Cell Differentiation, Neovascularization, and Angiogenesis #MMPMID28207177
Cochrane A; Kelaini S; Tsifaki M; Bojdo J; Vilŕ?González M; Drehmer D; Caines R; Magee C; Eleftheriadou M; Hu Y; Grieve D; Stitt AW; Zeng L; Xu Q; Margariti A
Stem Cells 2017[Apr]; 35 (4): 952-66 PMID28207177show ga
The capability to derive endothelial cell (ECs) from induced pluripotent stem cells (iPSCs) holds huge therapeutic potential for cardiovascular disease. This study elucidates the precise role of the RNA?binding protein Quaking isoform 5 (QKI?5) during EC differentiation from both mouse and human iPSCs (hiPSCs) and dissects how RNA?binding proteins can improve differentiation efficiency toward cell therapy for important vascular diseases. iPSCs represent an attractive cellular approach for regenerative medicine today as they can be used to generate patient?specific therapeutic cells toward autologous cell therapy. In this study, using the model of iPSCs differentiation toward ECs, the QKI?5 was found to be an important regulator of STAT3 stabilization and vascular endothelial growth factor receptor 2 (VEGFR2) activation during the EC differentiation process. QKI?5 was induced during EC differentiation, resulting in stabilization of STAT3 expression and modulation of VEGFR2 transcriptional activation as well as VEGF secretion through direct binding to the 3? UTR of STAT3. Importantly, mouse iPS?ECs overexpressing QKI?5 significantly improved angiogenesis and neovascularization and blood flow recovery in experimental hind limb ischemia. Notably, hiPSCs overexpressing QKI?5, induced angiogenesis on Matrigel plug assays in vivo only 7 days after subcutaneous injection in SCID mice. These results highlight a clear functional benefit of QKI?5 in neovascularization, blood flow recovery, and angiogenesis. Thus, they provide support to the growing consensus that elucidation of the molecular mechanisms underlying EC differentiation will ultimately advance stem cell regenerative therapy and eventually make the treatment of cardiovascular disease a reality. The RNA binding protein QKI?5 is induced during EC differentiation from iPSCs. RNA binding protein QKI?5 was induced during EC differentiation in parallel with the EC marker CD144. Immunofluorescence staining showing that QKI?5 is localized in the nucleus and stained in parallel with CD144 in differentiated ECs (scale bar?=?50 µm). stemcells2017 Stem Cells2017;35:952?966
free
free
free
Stem+Cells 2017 ; 35 (4): 952-66
