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10.1002/1873-3468.12469 http://scihub22266oqcxt.onion/10.1002/1873-3468.12469 C5396285!5396285!27800615     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27800615&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       FEBS+Lett 2016 ; 590 (23): 4343-53 Nephropedia Template TP {{tp|p=27800615|t=2016. Simultaneous generation of multi?gene knockouts in human cells |pdf=|usr=}}{{27800615}} gab.com Text Simultaneous generation of multi gene knockouts in human cells JO: FEBS Lett http://www.kidney.de/pget.php?&tw=1&pmid=27800615 #FOAvip Genome?editing techniques enable the generation of gene knockouts in various mammalian cell lines. However, it remains technically challenging to completely disrupt a targeted gene using a canonical method in a timely manner. To improve the efficiency of producing reliable genomic modifications, we designed a method using a linear donor fragment containing a reporter system. Combined with a homologous recombination?independent knock?in strategy, we successfully enriched those cell clones that specifically carry the target gene mutations. We observed a much improved success rate when generating single? and multiple?gene knockouts in a one?step procedure using this special protocol coupled with the CRISPR/Cas9 system. This new approach further empowers the molecular biological study of genes and their functions. Twit Text FOAVip Simultaneous generation of multi gene knockouts in human cells ????FEBS Lett http://www.kidney.de/pget.php?&tw=1&pmid=27800615 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27800615 #FOAvip English Wikipedia <ref>{{Cite pmid|27800615}}</ref> Simultaneous generation of multi?gene knockouts in human cells #MMPMID27800615 Zhou Y; Zhang H; Wei W FEBS Lett 2016[Dec]; 590 (23): 4343-53 PMID27800615show ga Genome?editing techniques enable the generation of gene knockouts in various mammalian cell lines. However, it remains technically challenging to completely disrupt a targeted gene using a canonical method in a timely manner. To improve the efficiency of producing reliable genomic modifications, we designed a method using a linear donor fragment containing a reporter system. Combined with a homologous recombination?independent knock?in strategy, we successfully enriched those cell clones that specifically carry the target gene mutations. We observed a much improved success rate when generating single? and multiple?gene knockouts in a one?step procedure using this special protocol coupled with the CRISPR/Cas9 system. This new approach further empowers the molecular biological study of genes and their functions. äSimultaneous generation of multi?gene knockouts in human cells (epmc).pdf DeepDyve Pubget Overpricing