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10.1002/ece3.2893 http://scihub22266oqcxt.onion/10.1002/ece3.2893 C5395458!5395458!28428873     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Ecol+Evol 2017 ; 7 (8): 2835-45 Nephropedia Template TP {{tp|p=28428873|t=2017. A maize landrace that emits defense volatiles in response to herbivore eggs possesses a strongly inducible terpene synthase gene |pdf=|usr=}}{{28428873}} gab.com Text A maize landrace that emits defense volatiles in response to herbivore eggs possesses a strongly inducible terpene synthase gene JO: Ecol Evol http://www.kidney.de/pget.php?&tw=1&pmid=28428873 #FOAvip Maize (Zea mays) emits volatile terpenes in response to insect feeding and egg deposition to defend itself against harmful pests. However, maize cultivars differ strongly in their ability to produce the defense signal. To further understand the agroecological role and underlying genetic mechanisms for variation in terpene emission among maize cultivars, we studied the production of an important signaling component (E)?caryophyllene in a South American maize landrace Braz1006 possessing stemborer Chilo partellus egg inducible defense trait, in comparison with the European maize line Delprim and North American inbred line B73. The (E)?caryophyllene production level and transcript abundance of TPS23, terpene synthase responsible for (E)?caryophyllene formation, were compared between Braz1006, Delprim, and B73 after mimicked herbivory. Braz1006?TPS23 was heterologously expressed in E. coli, and amino acid sequences were determined. Furthermore, electrophysiological and behavioral responses of a key parasitic wasp Cotesia sesamiae to C. partellus egg?induced Braz1006 volatiles were determined using coupled gas chromatography electroantennography and olfactometer bioassay studies. After elicitor treatment, Braz1006 released eightfold higher (E)?caryophyllene than Delprim, whereas no (E)?caryophyllene was detected in B73. The superior (E)?caryophyllene production by Braz1006 was positively correlated with high transcript levels of TPS23 in the landrace compared to Delprim. TPS23 alleles from Braz1006 showed dissimilarities at different sequence positions with Delprim and B73 and encodes an active enzyme. Cotesia sesamiae was attracted to egg?induced volatiles from Braz1006 and synthetic (E)?caryophyllene. The variation in (E)?caryophyllene emission between Braz1006 and Delprim is positively correlated with induced levels of TPS23 transcripts. The enhanced TPS23 activity and corresponding (E)?caryophyllene production by the maize landrace could be attributed to the differences in amino acid sequence with the other maize lines. This study suggested that the same analogous genes could have contrasting expression patterns in different maize genetic backgrounds. The current findings provide valuable insight not only into genetic mechanisms underlying variation in defense signal production but also the prospect of introgressing the novel defense traits into elite maize varieties for effective and ecologically sound protection of crops against damaging insect pests. Twit Text FOAVip A maize landrace that emits defense volatiles in response to herbivore eggs possesses a strongly inducible terpene synthase gene ????Ecol Evol http://www.kidney.de/pget.php?&tw=1&pmid=28428873 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28428873 #FOAvip English Wikipedia <ref>{{Cite pmid|28428873}}</ref> A maize landrace that emits defense volatiles in response to herbivore eggs possesses a strongly inducible terpene synthase gene #MMPMID28428873 Tamiru A; Bruce TJA; Richter A; Woodcock CM; Midega CAO; Degenhardt J; Kelemu S; Pickett JA; Khan ZR Ecol Evol 2017[Apr]; 7 (8): 2835-45 PMID28428873show ga Maize (Zea mays) emits volatile terpenes in response to insect feeding and egg deposition to defend itself against harmful pests. However, maize cultivars differ strongly in their ability to produce the defense signal. To further understand the agroecological role and underlying genetic mechanisms for variation in terpene emission among maize cultivars, we studied the production of an important signaling component (E)?caryophyllene in a South American maize landrace Braz1006 possessing stemborer Chilo partellus egg inducible defense trait, in comparison with the European maize line Delprim and North American inbred line B73. The (E)?caryophyllene production level and transcript abundance of TPS23, terpene synthase responsible for (E)?caryophyllene formation, were compared between Braz1006, Delprim, and B73 after mimicked herbivory. Braz1006?TPS23 was heterologously expressed in E. coli, and amino acid sequences were determined. Furthermore, electrophysiological and behavioral responses of a key parasitic wasp Cotesia sesamiae to C. partellus egg?induced Braz1006 volatiles were determined using coupled gas chromatography electroantennography and olfactometer bioassay studies. After elicitor treatment, Braz1006 released eightfold higher (E)?caryophyllene than Delprim, whereas no (E)?caryophyllene was detected in B73. The superior (E)?caryophyllene production by Braz1006 was positively correlated with high transcript levels of TPS23 in the landrace compared to Delprim. TPS23 alleles from Braz1006 showed dissimilarities at different sequence positions with Delprim and B73 and encodes an active enzyme. Cotesia sesamiae was attracted to egg?induced volatiles from Braz1006 and synthetic (E)?caryophyllene. The variation in (E)?caryophyllene emission between Braz1006 and Delprim is positively correlated with induced levels of TPS23 transcripts. The enhanced TPS23 activity and corresponding (E)?caryophyllene production by the maize landrace could be attributed to the differences in amino acid sequence with the other maize lines. This study suggested that the same analogous genes could have contrasting expression patterns in different maize genetic backgrounds. The current findings provide valuable insight not only into genetic mechanisms underlying variation in defense signal production but also the prospect of introgressing the novel defense traits into elite maize varieties for effective and ecologically sound protection of crops against damaging insect pests. äA maize landrace that emits defense volatiles in response to herbivore(epmc).pdf DeepDyve Pubget Overpricing