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10.1242/jcs.188433 http://scihub22266oqcxt.onion/10.1242/jcs.188433 C5394779!5394779 !27445312     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27445312 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Cell+Sci 2017 ; 130 (1 ): 278-291 Nephropedia Template TP {{tp|p=27445312 |t=2017. 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy |pdf=|usr=}}{{27445312 }} gab.com Text 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy JO: J Cell Sci http://www.kidney.de/pget.php?&tw=1&pmid=27445312 #FOAvip The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastructure. The workflow encompasses all steps from cell culture to sample processing, imaging strategy, and 3D image processing and analysis. We demonstrate successful application of the workflow to three studies, each aiming to better understand complex and dynamic biological processes, including bacterial and viral infections of cultured cells and formation of entotic cell-in-cell structures commonly observed in tumours. Our workflow revealed new insight into the replicative niche of Mycobacterium tuberculosis in primary human lymphatic endothelial cells, HIV-1 in human monocyte-derived macrophages, and the composition of the entotic vacuole. The broad application of this 3D CLEM technique will make it a useful addition to the correlative imaging toolbox for biomedical research. Twit Text FOAVip 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy ????J Cell Sci http://www.kidney.de/pget.php?&tw=1&pmid=27445312 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27445312 #FOAvip English Wikipedia <ref>{{Cite pmid|27445312 }}</ref> 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy #MMPMID27445312 Russell MR ; Lerner TR ; Burden JJ ; Nkwe DO ; Pelchen-Matthews A ; Domart MC ; Durgan J ; Weston A ; Jones ML ; Peddie CJ ; Carzaniga R ; Florey O ; Marsh M ; Gutierrez MG ; Collinson LM J Cell Sci 2017[Jan]; 130 (1 ): 278-291 PMID27445312 show ga The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastructure. The workflow encompasses all steps from cell culture to sample processing, imaging strategy, and 3D image processing and analysis. We demonstrate successful application of the workflow to three studies, each aiming to better understand complex and dynamic biological processes, including bacterial and viral infections of cultured cells and formation of entotic cell-in-cell structures commonly observed in tumours. Our workflow revealed new insight into the replicative niche of Mycobacterium tuberculosis in primary human lymphatic endothelial cells, HIV-1 in human monocyte-derived macrophages, and the composition of the entotic vacuole. The broad application of this 3D CLEM technique will make it a useful addition to the correlative imaging toolbox for biomedical research. |*Imaging, Three-Dimensional [MESH] |Cell Survival [MESH] |Cells, Cultured [MESH] |Endothelial Cells/microbiology/*ultrastructure [MESH] |Entosis [MESH] |HIV/ultrastructure [MESH] |Humans [MESH] |Intracellular Space/microbiology [MESH] |Macrophages/*ultrastructure/virology [MESH] |Microscopy, Electron, Scanning/*methods [MESH] |Monocytes/cytology [MESH]3D correlative light and electron microscopy of cultured cells using s(epmc).pdf DeepDyve Pubget Overpricing