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2017 ; 18
(12
): 2991-3004
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English Wikipedia
Tissue Myeloid Progenitors Differentiate into Pericytes through TGF-? Signaling
in Developing Skin Vasculature
#MMPMID28329690
Yamazaki T
; Nalbandian A
; Uchida Y
; Li W
; Arnold TD
; Kubota Y
; Yamamoto S
; Ema M
; Mukouyama YS
Cell Rep
2017[Mar]; 18
(12
): 2991-3004
PMID28329690
show ga
Mural cells (pericytes and vascular smooth muscle cells) are essential for the
regulation of vascular networks and maintenance of vascular integrity, but their
origins are diverse in different tissues and not known in the organs that arise
from the ectoderm, such as skin. Here, we show that tissue-localized myeloid
progenitors contribute to pericyte development in embryonic skin vasculature. A
series of in vivo fate-mapping experiments indicates that tissue myeloid
progenitors differentiate into pericytes. Furthermore, depletion of tissue
myeloid cells and their progenitors in PU.1 (also known as Spi1) mutants results
in defective pericyte development. Fluorescence-activated cell sorting
(FACS)-isolated myeloid cells and their progenitors from embryonic skin
differentiate into pericytes in culture. At the molecular level, transforming
growth factor-? (TGF-?) induces pericyte differentiation in culture. Furthermore,
type 2 TGF-? receptor (Tgfbr2) mutants exhibit deficient pericyte development in
skin vasculature. Combined, these data suggest that pericytes differentiate from
tissue myeloid progenitors in the skin vasculature through TGF-? signaling.