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10.1210/en.2015-1636 http://scihub22266oqcxt.onion/10.1210/en.2015-1636 C5393343!5393343 !26431226     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26431226 &cmd=llinks): Failed to open stream: HTTP request failed! 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Development of an Enhanced Sensitivity Bead-Based Immunoassay for Real-Time In Vivo Detection of Pancreatic ?-Cell Death |pdf=|usr=}}{{26431226 }} gab.com Text Development of an Enhanced Sensitivity Bead-Based Immunoassay for Real-Time In Vivo Detection of Pancreatic -Cell Death JO: Endocrinology http://www.kidney.de/pget.php?&tw=1&pmid=26431226 #FOAvip There is a clinical need for plasma tests to detect and quantify the in vivo destruction of pancreatic ?-cells in type 1 diabetes. We previously developed a time-resolved fluorescence immunoassay (TRFIA) to glutamate decarboxylase 65 kDa (GAD65) (GAD65-TRFIA) that was able to detect the synchronous necrotic destruction of transplanted ?-cells in the hours after their infusion in the liver. This GAD65-TRFIA, however, lacked sensitivity to detect continued ?-cell rejection beyond this acute phase. The aim of present study was to gain at least an order of magnitude in analytical sensitivity by switching to Becton Dickinson cytometric bead array (CBA) (GAD65-CBA) enhanced sensitivity format, using the same couple of monoclonal antibodies. We compared the performances of GAD65-CBA and GAD65-TRFIA using Clinical and Laboratory Standards Institute protocols for linearity, imprecision, specificity, limit of detection, and functional sensitivity. We conducted a method comparison and assessed the biologic potential on samples from human recipients of islet grafts. The GAD65-CBA showed acceptable linearity and imprecision. Switching from TRFIA to CBA lowered functional sensitivity by a factor 35 and lowered limit of detection by a factor 11 with minimal need for method optimization. The enhanced sensitivity greatly expands the application domain of our biomarker and allowed for the first time to detect ongoing ?-cell destruction up to at least 1 day after islet transplantation. We conclude that the GAD65-CBA is suitable for biological and clinical assessment of the real-time destruction of ?-cells in intraportal transplantation. Twit Text FOAVip Development of an Enhanced Sensitivity Bead-Based Immunoassay for Real-Time In Vivo Detection of Pancreatic -Cell Death ????Endocrinology http://www.kidney.de/pget.php?&tw=1&pmid=26431226 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26431226 #FOAvip English Wikipedia <ref>{{Cite pmid|26431226 }}</ref> Development of an Enhanced Sensitivity Bead-Based Immunoassay for Real-Time In Vivo Detection of Pancreatic ?-Cell Death #MMPMID26431226 Costa OR ; Stangé G ; Verhaeghen K ; Brackeva B ; Nonneman E ; Hampe CS ; Ling Z ; Pipeleers D ; Gorus FK ; Martens GA Endocrinology 2015[Dec]; 156 (12 ): 4755-60 PMID26431226 show ga There is a clinical need for plasma tests to detect and quantify the in vivo destruction of pancreatic ?-cells in type 1 diabetes. We previously developed a time-resolved fluorescence immunoassay (TRFIA) to glutamate decarboxylase 65 kDa (GAD65) (GAD65-TRFIA) that was able to detect the synchronous necrotic destruction of transplanted ?-cells in the hours after their infusion in the liver. This GAD65-TRFIA, however, lacked sensitivity to detect continued ?-cell rejection beyond this acute phase. The aim of present study was to gain at least an order of magnitude in analytical sensitivity by switching to Becton Dickinson cytometric bead array (CBA) (GAD65-CBA) enhanced sensitivity format, using the same couple of monoclonal antibodies. We compared the performances of GAD65-CBA and GAD65-TRFIA using Clinical and Laboratory Standards Institute protocols for linearity, imprecision, specificity, limit of detection, and functional sensitivity. We conducted a method comparison and assessed the biologic potential on samples from human recipients of islet grafts. The GAD65-CBA showed acceptable linearity and imprecision. Switching from TRFIA to CBA lowered functional sensitivity by a factor 35 and lowered limit of detection by a factor 11 with minimal need for method optimization. The enhanced sensitivity greatly expands the application domain of our biomarker and allowed for the first time to detect ongoing ?-cell destruction up to at least 1 day after islet transplantation. We conclude that the GAD65-CBA is suitable for biological and clinical assessment of the real-time destruction of ?-cells in intraportal transplantation. |*Apoptosis [MESH] |*Islets of Langerhans Transplantation [MESH] |Biomarkers/blood [MESH] |Diabetes Mellitus, Type 1/*blood/therapy [MESH] |Fluoroimmunoassay/methods [MESH] |Glutamate Decarboxylase/*blood [MESH] |Humans [MESH] |Immunoassay/methods [MESH] |Insulin-Secreting Cells/*metabolism [MESH]Development of an Enhanced Sensitivity Bead-Based Immunoassay for Real(epmc).pdf DeepDyve Pubget Overpricing