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10.1101/gad.294413.116 http://scihub22266oqcxt.onion/10.1101/gad.294413.116 C5393050!5393050 !28404629     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28404629 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Genes+Dev 2017 ; 31 (6 ): 537-552 Nephropedia Template TP {{tp|p=28404629 |t=2017. BRaf signaling principles unveiled by large-scale human mutation analysis with a rapid lentivirus-based gene replacement method |pdf=|usr=}}{{28404629 }} gab.com Text BRaf signaling principles unveiled by large-scale human mutation analysis with a rapid lentivirus-based gene replacement method JO: Genes Dev http://www.kidney.de/pget.php?&tw=1&pmid=28404629 #FOAvip Rapid advances in genetics are linking mutations on genes to diseases at an exponential rate, yet characterizing the gene-mutation-cell-behavior relationships essential for precision medicine remains a daunting task. More than 350 mutations on small GTPase BRaf are associated with various tumors, and ?40 mutations are associated with the neurodevelopmental disorder cardio-facio-cutaneous syndrome (CFC). We developed a fast cost-effective lentivirus-based rapid gene replacement method to interrogate the physiopathology of BRaf and ?50 disease-linked BRaf mutants, including all CFC-linked mutants. Analysis of simultaneous multiple patch-clamp recordings from 6068 pairs of rat neurons with validation in additional mouse and human neurons and multiple learning tests from 1486 rats identified BRaf as the key missing signaling effector in the common synaptic NMDA-R-CaMKII-SynGap-Ras-BRaf-MEK-ERK transduction cascade. Moreover, the analysis creates the original big data unveiling three general features of BRaf signaling. This study establishes the first efficient procedure that permits large-scale functional analysis of human disease-linked mutations essential for precision medicine. Twit Text FOAVip BRaf signaling principles unveiled by large-scale human mutation analysis with a rapid lentivirus-based gene replacement method ????Genes Dev http://www.kidney.de/pget.php?&tw=1&pmid=28404629 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28404629 #FOAvip English Wikipedia <ref>{{Cite pmid|28404629 }}</ref> BRaf signaling principles unveiled by large-scale human mutation analysis with a rapid lentivirus-based gene replacement method #MMPMID28404629 Lim CS ; Kang X ; Mirabella V ; Zhang H ; Bu Q ; Araki Y ; Hoang ET ; Wang S ; Shen Y ; Choi S ; Kaang BK ; Chang Q ; Pang ZP ; Huganir RL ; Zhu JJ Genes Dev 2017[Mar]; 31 (6 ): 537-552 PMID28404629 show ga Rapid advances in genetics are linking mutations on genes to diseases at an exponential rate, yet characterizing the gene-mutation-cell-behavior relationships essential for precision medicine remains a daunting task. More than 350 mutations on small GTPase BRaf are associated with various tumors, and ?40 mutations are associated with the neurodevelopmental disorder cardio-facio-cutaneous syndrome (CFC). We developed a fast cost-effective lentivirus-based rapid gene replacement method to interrogate the physiopathology of BRaf and ?50 disease-linked BRaf mutants, including all CFC-linked mutants. Analysis of simultaneous multiple patch-clamp recordings from 6068 pairs of rat neurons with validation in additional mouse and human neurons and multiple learning tests from 1486 rats identified BRaf as the key missing signaling effector in the common synaptic NMDA-R-CaMKII-SynGap-Ras-BRaf-MEK-ERK transduction cascade. Moreover, the analysis creates the original big data unveiling three general features of BRaf signaling. This study establishes the first efficient procedure that permits large-scale functional analysis of human disease-linked mutations essential for precision medicine. |*Mutation [MESH] |Animals [MESH] |Cells, Cultured [MESH] |Disease/genetics [MESH] |Female [MESH] |Gene Transfer Techniques [MESH] |Humans [MESH] |Lentivirus/genetics [MESH] |MAP Kinase Signaling System/*genetics [MESH] |Male [MESH] |Mice, Inbred C57BL [MESH] |Neurons/physiology [MESH] |Proto-Oncogene Proteins B-raf/*genetics [MESH] |Rats, Sprague-Dawley [MESH] |Synaptic Transmission/*genetics [MESH]BRaf signaling principles unveiled by large-scale human mutation analy(epmc).pdf DeepDyve Pubget Overpricing