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10.1186/s13062-017-0180-7 http://scihub22266oqcxt.onion/10.1186/s13062-017-0180-7 C5392957!5392957 !28412966     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28412966 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biol+Direct 2017 ; 12 (1 ): 8 Nephropedia Template TP {{tp|p=28412966 |t=2017. TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induced epithelial to mesenchymal transition |pdf=|usr=}}{{28412966 }} gab.com Text TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induced epithelial to mesenchymal transition JO: Biol Direct http://www.kidney.de/pget.php?&tw=1&pmid=28412966 #FOAvip BACKGROUND: The transition between epithelial and mesenchymal phenotypes (EMT) occurs in a variety of contexts. It is critical for mammalian development and it is also involved in tumor initiation and progression. Master transcription factor (TF) regulators of this process are conserved between mouse and human. METHODS: From a computational analysis of a variety of high-throughput sequencing data sets we initially inferred that TFAP2A is connected to the core EMT network in both species. We then analysed publicly available human breast cancer data for TFAP2A expression and also studied the expression (by mRNA sequencing), activity (by monitoring the expression of its predicted targets), and binding (by electrophoretic mobility shift assay and chromatin immunoprecipitation) of this factor in a mouse mammary gland EMT model system (NMuMG) cell line. RESULTS: We found that upon induction of EMT, the activity of TFAP2A, reflected in the expression level of its predicted targets, is up-regulated in a variety of systems, both murine and human, while TFAP2A's expression is increased in more "stem-like" cancers. We provide strong evidence for the direct interaction between the TFAP2A TF and the ZEB2 promoter and we demonstrate that this interaction affects ZEB2 expression. Overexpression of TFAP2A from an exogenous construct perturbs EMT, however, in a manner similar to the downregulation of endogenous TFAP2A that takes place during EMT. CONCLUSIONS: Our study reveals that TFAP2A is a conserved component of the core network that regulates EMT, acting as a repressor of many genes, including ZEB2. REVIEWERS: This article has been reviewed by Dr. Martijn Huynen and Dr. Nicola Aceto. Twit Text FOAVip TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induced epithelial to mesenchymal transition ????Biol Direct http://www.kidney.de/pget.php?&tw=1&pmid=28412966 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28412966 #FOAvip English Wikipedia <ref>{{Cite pmid|28412966 }}</ref> TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induced epithelial to mesenchymal transition #MMPMID28412966 Dimitrova Y ; Gruber AJ ; Mittal N ; Ghosh S ; Dimitriades B ; Mathow D ; Grandy WA ; Christofori G ; Zavolan M Biol Direct 2017[Apr]; 12 (1 ): 8 PMID28412966 show ga BACKGROUND: The transition between epithelial and mesenchymal phenotypes (EMT) occurs in a variety of contexts. It is critical for mammalian development and it is also involved in tumor initiation and progression. Master transcription factor (TF) regulators of this process are conserved between mouse and human. METHODS: From a computational analysis of a variety of high-throughput sequencing data sets we initially inferred that TFAP2A is connected to the core EMT network in both species. We then analysed publicly available human breast cancer data for TFAP2A expression and also studied the expression (by mRNA sequencing), activity (by monitoring the expression of its predicted targets), and binding (by electrophoretic mobility shift assay and chromatin immunoprecipitation) of this factor in a mouse mammary gland EMT model system (NMuMG) cell line. RESULTS: We found that upon induction of EMT, the activity of TFAP2A, reflected in the expression level of its predicted targets, is up-regulated in a variety of systems, both murine and human, while TFAP2A's expression is increased in more "stem-like" cancers. We provide strong evidence for the direct interaction between the TFAP2A TF and the ZEB2 promoter and we demonstrate that this interaction affects ZEB2 expression. Overexpression of TFAP2A from an exogenous construct perturbs EMT, however, in a manner similar to the downregulation of endogenous TFAP2A that takes place during EMT. CONCLUSIONS: Our study reveals that TFAP2A is a conserved component of the core network that regulates EMT, acting as a repressor of many genes, including ZEB2. REVIEWERS: This article has been reviewed by Dr. Martijn Huynen and Dr. Nicola Aceto. |*Gene Expression Regulation, Neoplastic [MESH] |Animals [MESH] |Cell Line [MESH] |Epithelial-Mesenchymal Transition/*genetics [MESH] |Female [MESH] |Homeodomain Proteins/*genetics/metabolism [MESH] |Humans [MESH] |Mammary Glands, Animal/metabolism/physiopathology [MESH] |Mice [MESH] |Repressor Proteins/*genetics/metabolism [MESH] |Transcription Factor AP-2/genetics/*metabolism [MESH] |Transforming Growth Factor beta1/genetics/metabolism [MESH] |Zinc Finger E-box Binding Homeobox 2 [MESH]TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induc(epmc).pdf DeepDyve Pubget Overpricing