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10.7554/eLife.23588 http://scihub22266oqcxt.onion/10.7554/eLife.23588 C5391207!5391207!28323620     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       eLife 2017 ; 6 (ä): ä Nephropedia Template TP {{tp|p=28323620|t=2017. Discovery of novel determinants of endothelial lineage using chimeric heterokaryons |pdf=|usr=}}{{28323620}} gab.com Text Discovery of novel determinants of endothelial lineage using chimeric heterokaryons JO: eLife http://www.kidney.de/pget.php?&tw=1&pmid=28323620 #FOAvip We wish to identify determinants of endothelial lineage. Murine embryonic stem cells (mESC) were fused with human endothelial cells in stable, non-dividing, heterokaryons. Using RNA-seq, it is possible to discriminate between human and mouse transcripts in these chimeric heterokaryons. We observed a temporal pattern of gene expression in the ESCs of the heterokaryons that recapitulated ontogeny, with early mesodermal factors being expressed before mature endothelial genes. A set of transcriptional factors not known to be involved in endothelial development was upregulated, one of which was POU class 3 homeobox 2 (Pou3f2). We confirmed its importance in differentiation to endothelial lineage via loss- and gain-of-function (LOF and GOF). Its role in vascular development was validated in zebrafish embryos using morpholino oligonucleotides. These studies provide a systematic and mechanistic approach for identifying key regulators in directed differentiation of pluripotent stem cells to somatic cell lineages.DOI:http://dx.doi.org/10.7554/eLife.23588.001 Twit Text FOAVip Discovery of novel determinants of endothelial lineage using chimeric heterokaryons ????eLife http://www.kidney.de/pget.php?&tw=1&pmid=28323620 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28323620 #FOAvip English Wikipedia <ref>{{Cite pmid|28323620}}</ref> Discovery of novel determinants of endothelial lineage using chimeric heterokaryons #MMPMID28323620 Wong WT; Matrone G; Tian X; Tomoiaga SA; Au KF; Meng S; Yamazoe S; Sieveking D; Chen K; Burns DM; Chen JK; Blau HM; Cooke JP eLife 2017[]; 6 (ä): ä PMID28323620show ga We wish to identify determinants of endothelial lineage. Murine embryonic stem cells (mESC) were fused with human endothelial cells in stable, non-dividing, heterokaryons. Using RNA-seq, it is possible to discriminate between human and mouse transcripts in these chimeric heterokaryons. We observed a temporal pattern of gene expression in the ESCs of the heterokaryons that recapitulated ontogeny, with early mesodermal factors being expressed before mature endothelial genes. A set of transcriptional factors not known to be involved in endothelial development was upregulated, one of which was POU class 3 homeobox 2 (Pou3f2). We confirmed its importance in differentiation to endothelial lineage via loss- and gain-of-function (LOF and GOF). Its role in vascular development was validated in zebrafish embryos using morpholino oligonucleotides. These studies provide a systematic and mechanistic approach for identifying key regulators in directed differentiation of pluripotent stem cells to somatic cell lineages.DOI:http://dx.doi.org/10.7554/eLife.23588.001 äDiscovery of novel determinants of endothelial lineage using chimeric (epmc).pdf DeepDyve Pubget Overpricing