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Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Methods+Appl+Fluoresc 2016 ; 4 (2): ä Nephropedia Template TP
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From single-molecule spectroscopy to super-resolution imaging of the neuron: a review #MMPMID28809165
Laine RF; Kaminski Schierle GS; van de Linde S; Kaminski CF
Methods Appl Fluoresc 2016[Jun]; 4 (2): ä PMID28809165show ga
For more than 20 years, single-molecule spectroscopy has been providing invaluable insights into nature at the molecular level. The field has received a powerful boost with the development of the technique into super-resolution imaging methods, ca. 10 years ago, which overcome the limitations imposed by optical diffraction. Today, single molecule super-resolution imaging is routinely used in the study of macromolecular function and structure in the cell. Concomitantly, computational methods have been developed that provide information on numbers and positions of molecules at the nanometer-scale. In this overview, we outline the technical developments that have led to the emergence of localization microscopy techniques from single-molecule spectroscopy. We then provide a comprehensive review on the application of the technique in the field of neuroscience research.