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Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 J+Physiol 2017 ; 595 (8): 2535-50 Nephropedia Template TP
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Endothelin?1 mediates natriuresis but not polyuria during vitamin D?induced acute hypercalcaemia #MMPMID28120456
J Physiol 2017[Apr]; 595 (8): 2535-50 PMID28120456show ga
Key points: Hypercalcaemia can occur under various pathological conditions, such as primary hyperparathyroidism, malignancy or granulomatosis, and it induces natriuresis and polyuria in various species via an unknown mechanism.A previous study demonstrated that hypercalcaemia induced by vitamin D in rats increased endothelin (ET)?1 expression in the distal nephron, which suggests the involvement of the ET system in hypercalcaemia?induced effects.In the present study, we demonstrate that, during vitamin D?induced hypercalcaemia, the activation of ET system by increased ET?1 is responsible for natriuresis but not for polyuria.Vitamin D?treated hypercalcaemic mice showed a blunted response to amiloride, suggesting that epithelial sodium channel function is inhibited.We have identified an original pathway that specifically mediates the effects of vitamin D?induced hypercalcaemia on sodium handling in the distal nephron without affecting water handling. Abstract: Acute hypercalcaemia increases urinary sodium and water excretion; however, the underlying molecular mechanism remains unclear. Because vitamin D?induced hypercalcaemia increases the renal expression of endothelin (ET)?1, we hypothesized that ET?1 mediates the effects of hypercalcaemia on renal sodium and water handling. Hypercalcaemia was induced in 8?week?old, parathyroid hormone?supplemented, male mice by oral administration of dihydrotachysterol (DHT) for 3 days. DHT?treated mice became hypercalcaemic and displayed increased urinary water and sodium excretion compared to controls. mRNA levels of ET?1 and the transcription factors CCAAT?enhancer binding protein ? and ? were specifically increased in the distal convoluted tubule and downstream segments in DHT?treated mice. To examine the role of the ET system in hypercalcaemia?induced natriuresis and polyuria, mice were treated with the ET?1 receptor antagonist macitentan, with or without DHT. Mice treated with both macitentan and DHT displayed hypercalcaemia and polyuria similar to that in mice treated with DHT alone; however, no increase in urinary sodium excretion was observed. To identify the affected sodium transport mechanism, we assessed the response to various diuretics in control and DHT?treated hypercalcaemic mice. Amiloride, an inhibitor of the epithelial sodium channel (ENaC), increased sodium excretion to a lesser extent in DHT?treated mice compared to control mice. Mice treated with either macitentan+DHT or macitentan alone had a similar response to amiloride. In summary, vitamin D?induced hypercalcaemia increases the renal production of ET?1 and decreases ENaC activity, which is probably responsible for the rise in urinary sodium excretion but not for polyuria.
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J+Physiol 2017 ; 595 (8): 2535-50
