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10.1007/7651_2016_9 http://scihub22266oqcxt.onion/10.1007/7651_2016_9 C5389928!5389928!27730537     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2019 ; 1576 (ä): 33-42 Nephropedia Template TP {{tp|p=27730537|t=2019. Construction of Thymus Organoids from Decellularized Thymus Scaffolds |pdf=|usr=}}{{27730537}} gab.com Text Construction of Thymus Organoids from Decellularized Thymus Scaffolds JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27730537 #FOAvip One of the hallmarks of modern medicine is the development of therapeutics that can modulate immune responses, especially the adaptive arm of immunity, for disease intervention and prevention. While tremendous progresses have been made in the past decades, manipulating the thymus, the primary lymphoid organ responsible for the development and education of T lymphocytes, remains a challenge. One of the major obstacles is the difficulty to reproduce its unique extracellular matrix (ECM) microenvironment that is essential for maintaining the function and survival of thymic epithelial cells (TECs), the predominant population of cells in the thymic stroma. Here, we described the construction of functional thymus organoids from decellularized thymus scaffolds repopulated with isolated TECs. Thymus decellularization was achieved by freeze/thaw cycles to induce intracellular ice crystal formation, followed by detergent-induced cell lysis. Cellular debris was removed with extensive wash. The decellularized thymus scaffolds can largely retain the 3-D extracellular matrix (ECM) microenvironment that can support the re-colonization of TECs. When transplanted into athymic nude mice, the reconstructed thymus organoids can effectively promote the homing of bone marrow-derived lymphocyte progenitors and support the development of a complex T cell repertoire. Bioengineering of thymus organoids can be a promising approach to rejuvenate/modulate the function of T-cell mediated adaptive immunity in regenerative medicine. Twit Text FOAVip Construction of Thymus Organoids from Decellularized Thymus Scaffolds ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27730537 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27730537 #FOAvip English Wikipedia <ref>{{Cite pmid|27730537}}</ref> Construction of Thymus Organoids from Decellularized Thymus Scaffolds #MMPMID27730537 Tajima A; Pradhan I; Geng X; Trucco M; Fan Y Methods Mol Biol 2019[]; 1576 (ä): 33-42 PMID27730537show ga One of the hallmarks of modern medicine is the development of therapeutics that can modulate immune responses, especially the adaptive arm of immunity, for disease intervention and prevention. While tremendous progresses have been made in the past decades, manipulating the thymus, the primary lymphoid organ responsible for the development and education of T lymphocytes, remains a challenge. One of the major obstacles is the difficulty to reproduce its unique extracellular matrix (ECM) microenvironment that is essential for maintaining the function and survival of thymic epithelial cells (TECs), the predominant population of cells in the thymic stroma. Here, we described the construction of functional thymus organoids from decellularized thymus scaffolds repopulated with isolated TECs. Thymus decellularization was achieved by freeze/thaw cycles to induce intracellular ice crystal formation, followed by detergent-induced cell lysis. Cellular debris was removed with extensive wash. The decellularized thymus scaffolds can largely retain the 3-D extracellular matrix (ECM) microenvironment that can support the re-colonization of TECs. When transplanted into athymic nude mice, the reconstructed thymus organoids can effectively promote the homing of bone marrow-derived lymphocyte progenitors and support the development of a complex T cell repertoire. Bioengineering of thymus organoids can be a promising approach to rejuvenate/modulate the function of T-cell mediated adaptive immunity in regenerative medicine. äConstruction of Thymus Organoids from Decellularized Thymus Scaffolds (epmc).pdf DeepDyve Pubget Overpricing