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10.1093/nar/gkw1171 http://scihub22266oqcxt.onion/10.1093/nar/gkw1171 C5389677!5389677!27903888     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2017 ; 45 (6): 3528-36 Nephropedia Template TP {{tp|p=27903888|t=2017. siRNA carrying an (E)-vinylphosphonate moiety at the 5? end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2 |pdf=|usr=}}{{27903888}} gab.com Text siRNA carrying an (E)-vinylphosphonate moiety at the 5 end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2 JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=27903888 #FOAvip Efficient gene silencing by RNA interference (RNAi) in vivo requires the recognition and binding of the 5?- phosphate of the guide strand of an siRNA by the Argonaute protein. However, for exogenous siRNAs it is limited by the rapid removal of the 5?- phosphate of the guide strand by metabolic enzymes. Here, we have determined the crystal structure of human Argonaute-2 in complex with the metabolically stable 5?-(E)-vinylphosphonate (5?-E-VP) guide RNA at 2.5-Å resolution. The structure demonstrates how the 5? binding site in the Mid domain of human Argonaute-2 is able to adjust the key residues in the 5?-nucleotide binding pocket to compensate for the change introduced by the modified nucleotide. This observation also explains improved binding affinity of the 5?-E-VP -modified siRNA to human Argonaute-2 in-vitro, as well as the enhanced silencing in the context of the trivalent N-acetylgalactosamine (GalNAc)-conjugated siRNA in mice relative to the un-modified siRNA. Twit Text FOAVip siRNA carrying an (E)-vinylphosphonate moiety at the 5 end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2 ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=27903888 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27903888 #FOAvip English Wikipedia <ref>{{Cite pmid|27903888}}</ref> siRNA carrying an (E)-vinylphosphonate moiety at the 5? end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2 #MMPMID27903888 Elkayam E; Parmar R; Brown CR; Willoughby JL; Theile CS; Manoharan M; Joshua-Tor L Nucleic Acids Res 2017[Apr]; 45 (6): 3528-36 PMID27903888show ga Efficient gene silencing by RNA interference (RNAi) in vivo requires the recognition and binding of the 5?- phosphate of the guide strand of an siRNA by the Argonaute protein. However, for exogenous siRNAs it is limited by the rapid removal of the 5?- phosphate of the guide strand by metabolic enzymes. Here, we have determined the crystal structure of human Argonaute-2 in complex with the metabolically stable 5?-(E)-vinylphosphonate (5?-E-VP) guide RNA at 2.5-Å resolution. The structure demonstrates how the 5? binding site in the Mid domain of human Argonaute-2 is able to adjust the key residues in the 5?-nucleotide binding pocket to compensate for the change introduced by the modified nucleotide. This observation also explains improved binding affinity of the 5?-E-VP -modified siRNA to human Argonaute-2 in-vitro, as well as the enhanced silencing in the context of the trivalent N-acetylgalactosamine (GalNAc)-conjugated siRNA in mice relative to the un-modified siRNA. äsiRNA carrying an (E)-vinylphosphonate moiety at the 5? end of the gui(epmc).pdf DeepDyve Pubget Overpricing