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2017 ; 45
(6
): 3116-3129
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Arabidopsis SWI/SNF chromatin remodeling complex binds both promoters and
terminators to regulate gene expression
#MMPMID27994035
Archacki R
; Yatusevich R
; Buszewicz D
; Krzyczmonik K
; Patryn J
; Iwanicka-Nowicka R
; Biecek P
; Wilczynski B
; Koblowska M
; Jerzmanowski A
; Swiezewski S
Nucleic Acids Res
2017[Apr]; 45
(6
): 3116-3129
PMID27994035
show ga
ATP-dependent chromatin remodeling complexes are important regulators of gene
expression in Eukaryotes. In plants, SWI/SNF-type complexes have been shown
critical for transcriptional control of key developmental processes, growth and
stress responses. To gain insight into mechanisms underlying these roles, we
performed whole genome mapping of the SWI/SNF catalytic subunit BRM in
Arabidopsis thaliana, combined with transcript profiling experiments. Our data
show that BRM occupies thousands of sites in Arabidopsis genome, most of which
located within or close to genes. Among identified direct BRM transcriptional
targets almost equal numbers were up- and downregulated upon BRM depletion,
suggesting that BRM can act as both activator and repressor of gene expression.
Interestingly, in addition to genes showing canonical pattern of BRM enrichment
near transcription start site, many other genes showed a transcription
termination site-centred BRM occupancy profile. We found that BRM-bound 3? gene
regions have promoter-like features, including presence of TATA boxes and high
H3K4me3 levels, and possess high antisense transcriptional activity which is
subjected to both activation and repression by SWI/SNF complex. Our data suggest
that binding to gene terminators and controlling transcription of non-coding RNAs
is another way through which SWI/SNF complex regulates expression of its targets.