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10.1093/nar/gkw1274 http://scihub22266oqcxt.onion/10.1093/nar/gkw1274 C5389615!5389615!27986854     free     free     free Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2017 ; 45 (4): 1983-93 Nephropedia Template TP {{tp|p=27986854|t=2017. A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction |pdf=|usr=}}{{27986854}} gab.com Text A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=27986854 #FOAvip The CRISPR (clustered regularly interspaced short palindromic repeats) system protects archaea and bacteria by eliminating nucleic acid invaders in a crRNA-guided manner. The Sulfolobus islandicus type III-B Cmr?? system targets invading nucleic acid at both RNA and DNA levels and DNA targeting relies on the directional transcription of the protospacer in vivo. To gain further insight into the involved mechanism, we purified a native effector complex of III-B Cmr?? from S. islandicus and characterized it in vitro. Cmr?? cleaved RNAs complementary to crRNA present in the complex and its ssDNA destruction activity was activated by target RNA. The ssDNA cleavage required mismatches between the 5?-tag of crRNA and the 3?-flanking region of target RNA. An invader plasmid assay showed that mutation either in the histidine-aspartate acid (HD) domain (a quadruple mutation) or in the GGDD motif of the Cmr?2? protein resulted in attenuation of the DNA interference in vivo. However, double mutation of the HD motif only abolished the DNase activity in vitro. Furthermore, the activated Cmr?? binary complex functioned as a highly active DNase to destroy a large excess DNA substrate, which could provide a powerful means to rapidly degrade replicating viral DNA. Twit Text FOAVip A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=27986854 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27986854 #FOAvip English Wikipedia <ref>{{Cite pmid|27986854}}</ref> A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction #MMPMID27986854 Han W; Li Y; Deng L; Feng M; Peng W; Hallstrøm S; Zhang J; Peng N; Liang YX; White MF; She Q Nucleic Acids Res 2017[Feb]; 45 (4): 1983-93 PMID27986854show ga The CRISPR (clustered regularly interspaced short palindromic repeats) system protects archaea and bacteria by eliminating nucleic acid invaders in a crRNA-guided manner. The Sulfolobus islandicus type III-B Cmr?? system targets invading nucleic acid at both RNA and DNA levels and DNA targeting relies on the directional transcription of the protospacer in vivo. To gain further insight into the involved mechanism, we purified a native effector complex of III-B Cmr?? from S. islandicus and characterized it in vitro. Cmr?? cleaved RNAs complementary to crRNA present in the complex and its ssDNA destruction activity was activated by target RNA. The ssDNA cleavage required mismatches between the 5?-tag of crRNA and the 3?-flanking region of target RNA. An invader plasmid assay showed that mutation either in the histidine-aspartate acid (HD) domain (a quadruple mutation) or in the GGDD motif of the Cmr?2? protein resulted in attenuation of the DNA interference in vivo. However, double mutation of the HD motif only abolished the DNase activity in vitro. Furthermore, the activated Cmr?? binary complex functioned as a highly active DNase to destroy a large excess DNA substrate, which could provide a powerful means to rapidly degrade replicating viral DNA. äA type III-B CRISPR-Cas effector complex mediating massive target DNA (epmc).pdf DeepDyve Pubget Overpricing