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10.1093/nar/gkw891 http://scihub22266oqcxt.onion/10.1093/nar/gkw891 C5389561!5389561!28204542     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2017 ; 45 (4): 2112-23 Nephropedia Template TP {{tp|p=28204542|t=2017. Molecular determinants for CRISPR RNA maturation in the Cas10?Csm complex and roles for non-Cas nucleases |pdf=|usr=}}{{28204542}} gab.com Text Molecular determinants for CRISPR RNA maturation in the Cas10 Csm complex and roles for non-Cas nucleases JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28204542 #FOAvip CRISPR?Cas (Clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) is a prokaryotic immune system that destroys foreign nucleic acids in a sequence-specific manner using Cas nucleases guided by short RNAs (crRNAs). Staphylococcus epidermidis harbours a Type III-A CRISPR?Cas system that encodes the Cas10?Csm interference complex and crRNAs that are subjected to multiple processing steps. The final step, called maturation, involves a concerted effort between Csm3, a ruler protein in Cas10?Csm that measures six-nucleotide increments, and the activity of a nuclease(s) that remains unknown. Here, we elucidate the contributions of the Cas10?Csm complex toward maturation and explore roles of non-Cas nucleases in this process. Using genetic and biochemical approaches, we show that charged residues in Csm3 facilitate its self-assembly and dictate the extent of maturation cleavage. Additionally, acidic residues in Csm5 are required for efficient maturation, but recombinant Csm5 fails to cleave crRNAs in vitro. However, we detected cellular nucleases that co-purify with Cas10?Csm, and show that Csm5 regulates their activities through distinct mechanisms. Altogether, our results support roles for non-Cas nuclease(s) during crRNA maturation and establish a link between Type III-A CRISPR?Cas immunity and central nucleic acid metabolism. Twit Text FOAVip Molecular determinants for CRISPR RNA maturation in the Cas10 Csm complex and roles for non-Cas nucleases ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28204542 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28204542 #FOAvip English Wikipedia <ref>{{Cite pmid|28204542}}</ref> Molecular determinants for CRISPR RNA maturation in the Cas10?Csm complex and roles for non-Cas nucleases #MMPMID28204542 Walker FC; Chou-Zheng L; Dunkle JA; Hatoum-Aslan A Nucleic Acids Res 2017[Feb]; 45 (4): 2112-23 PMID28204542show ga CRISPR?Cas (Clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) is a prokaryotic immune system that destroys foreign nucleic acids in a sequence-specific manner using Cas nucleases guided by short RNAs (crRNAs). Staphylococcus epidermidis harbours a Type III-A CRISPR?Cas system that encodes the Cas10?Csm interference complex and crRNAs that are subjected to multiple processing steps. The final step, called maturation, involves a concerted effort between Csm3, a ruler protein in Cas10?Csm that measures six-nucleotide increments, and the activity of a nuclease(s) that remains unknown. Here, we elucidate the contributions of the Cas10?Csm complex toward maturation and explore roles of non-Cas nucleases in this process. Using genetic and biochemical approaches, we show that charged residues in Csm3 facilitate its self-assembly and dictate the extent of maturation cleavage. Additionally, acidic residues in Csm5 are required for efficient maturation, but recombinant Csm5 fails to cleave crRNAs in vitro. However, we detected cellular nucleases that co-purify with Cas10?Csm, and show that Csm5 regulates their activities through distinct mechanisms. Altogether, our results support roles for non-Cas nuclease(s) during crRNA maturation and establish a link between Type III-A CRISPR?Cas immunity and central nucleic acid metabolism. äMolecular determinants for CRISPR RNA maturation in the Cas10?Csm comp(epmc).pdf DeepDyve Pubget Overpricing