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10.1093/nar/gkx042 http://scihub22266oqcxt.onion/10.1093/nar/gkx042 C5389539!5389539!28130424     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2017 ; 45 (4): 1946-57 Nephropedia Template TP {{tp|p=28130424|t=2017. The action of Escherichia coli CRISPR?Cas system on lytic bacteriophages with different lifestyles and development strategies |pdf=|usr=}}{{28130424}} gab.com Text The action of Escherichia coli CRISPR Cas system on lytic bacteriophages with different lifestyles and development strategies JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28130424 #FOAvip CRISPR?Cas systems provide prokaryotes with adaptive defense against bacteriophage infections. Given an enormous variety of strategies used by phages to overcome their hosts, one can expect that the efficiency of protective action of CRISPR?Cas systems against different viruses should vary. Here, we created a collection of Escherichia coli strains with type I-E CRISPR?Cas system targeting various positions in the genomes of bacteriophages ?, T5, T7, T4 and R1-37 and investigated the ability of these strains to resist the infection and acquire additional CRISPR spacers from the infecting phage. We find that the efficiency of CRISPR?Cas targeting by the host is determined by phage life style, the positions of the targeted protospacer within the genome, and the state of phage DNA. The results also suggest that during infection by lytic phages that are susceptible to CRISPR interference, CRISPR?Cas does not act as a true immunity system that saves the infected cell but rather enforces an abortive infection pathway leading to infected cell death with no phage progeny release. Twit Text FOAVip The action of Escherichia coli CRISPR Cas system on lytic bacteriophages with different lifestyles and development strategies ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28130424 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28130424 #FOAvip English Wikipedia <ref>{{Cite pmid|28130424}}</ref> The action of Escherichia coli CRISPR?Cas system on lytic bacteriophages with different lifestyles and development strategies #MMPMID28130424 Strotskaya A; Savitskaya E; Metlitskaya A; Morozova N; Datsenko KA; Semenova E; Severinov K Nucleic Acids Res 2017[Feb]; 45 (4): 1946-57 PMID28130424show ga CRISPR?Cas systems provide prokaryotes with adaptive defense against bacteriophage infections. Given an enormous variety of strategies used by phages to overcome their hosts, one can expect that the efficiency of protective action of CRISPR?Cas systems against different viruses should vary. Here, we created a collection of Escherichia coli strains with type I-E CRISPR?Cas system targeting various positions in the genomes of bacteriophages ?, T5, T7, T4 and R1-37 and investigated the ability of these strains to resist the infection and acquire additional CRISPR spacers from the infecting phage. We find that the efficiency of CRISPR?Cas targeting by the host is determined by phage life style, the positions of the targeted protospacer within the genome, and the state of phage DNA. The results also suggest that during infection by lytic phages that are susceptible to CRISPR interference, CRISPR?Cas does not act as a true immunity system that saves the infected cell but rather enforces an abortive infection pathway leading to infected cell death with no phage progeny release. äThe action of Escherichia coli CRISPR?Cas system on lytic bacteriophag(epmc).pdf DeepDyve Pubget Overpricing