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10.1093/nar/gkw911 http://scihub22266oqcxt.onion/10.1093/nar/gkw911 C5389476!5389476!27907896     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2017 ; 45 (5): 2797-808 Nephropedia Template TP {{tp|p=27907896|t=2017. Harnessing human ADAR2 for RNA repair ? Recoding a PINK1 mutation rescues mitophagy |pdf=|usr=}}{{27907896}} gab.com Text Harnessing human ADAR2 for RNA repair Recoding a PINK1 mutation rescues mitophagy JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=27907896 #FOAvip Site-directed A-to-I RNA editing is a technology for re-programming genetic information at the RNA-level. We describe here the first design of genetically encodable guideRNAs that enable the re-addressing of human ADAR2 toward specific sites in user-defined mRNA targets. Up to 65% editing yield has been achieved in cell culture for the recoding of a premature Stop codon (UAG) into tryptophan (UIG). In the targeted gene, editing was very specific. We applied the technology to recode a recessive loss-of-function mutation in PINK1 (W437X) in HeLa cells and showed functional rescue of PINK1/Parkin-mediated mitophagy, which is linked to the etiology of Parkinson's disease. In contrast to other editing strategies, this approach requires no artificial protein. Our novel guideRNAs may allow for the development of a platform technology that requires only the administration or expression of a guideRNA to recode genetic information, with high potential for application in biology and medicine. Twit Text FOAVip Harnessing human ADAR2 for RNA repair Recoding a PINK1 mutation rescues mitophagy ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=27907896 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27907896 #FOAvip English Wikipedia <ref>{{Cite pmid|27907896}}</ref> Harnessing human ADAR2 for RNA repair ? Recoding a PINK1 mutation rescues mitophagy #MMPMID27907896 Wettengel J; Reautschnig P; Geisler S; Kahle PJ; Stafforst T Nucleic Acids Res 2017[Mar]; 45 (5): 2797-808 PMID27907896show ga Site-directed A-to-I RNA editing is a technology for re-programming genetic information at the RNA-level. We describe here the first design of genetically encodable guideRNAs that enable the re-addressing of human ADAR2 toward specific sites in user-defined mRNA targets. Up to 65% editing yield has been achieved in cell culture for the recoding of a premature Stop codon (UAG) into tryptophan (UIG). In the targeted gene, editing was very specific. We applied the technology to recode a recessive loss-of-function mutation in PINK1 (W437X) in HeLa cells and showed functional rescue of PINK1/Parkin-mediated mitophagy, which is linked to the etiology of Parkinson's disease. In contrast to other editing strategies, this approach requires no artificial protein. Our novel guideRNAs may allow for the development of a platform technology that requires only the administration or expression of a guideRNA to recode genetic information, with high potential for application in biology and medicine. äHarnessing human ADAR2 for RNA repair ? Recoding a PINK1 mutation resc(epmc).pdf DeepDyve Pubget Overpricing