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10.7554/eLife.23001 http://scihub22266oqcxt.onion/10.7554/eLife.23001 C5388535!5388535!28290983     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       eLife 2017 ; 6 (ä): ä Nephropedia Template TP {{tp|p=28290983|t=2017. Total biosynthesis of the cyclic AMP booster forskolin from Coleus forskohlii |pdf=|usr=}}{{28290983}} gab.com Text Total biosynthesis of the cyclic AMP booster forskolin from Coleus forskohlii JO: eLife http://www.kidney.de/pget.php?&tw=1&pmid=28290983 #FOAvip Forskolin is a unique structurally complex labdane-type diterpenoid used in the treatment of glaucoma and heart failure based on its activity as a cyclic AMP booster. Commercial production of forskolin relies exclusively on extraction from its only known natural source, the plant Coleus forskohlii, in which forskolin accumulates in the root cork. Here, we report the discovery of five cytochrome P450s and two acetyltransferases which catalyze a cascade of reactions converting the forskolin precursor 13R-manoyl oxide into forskolin and a diverse array of additional labdane-type diterpenoids. A minimal set of three P450s in combination with a single acetyl transferase was identified that catalyzes the conversion of 13R-manoyl oxide into forskolin as demonstrated by transient expression in Nicotiana benthamiana. The entire pathway for forskolin production from glucose encompassing expression of nine genes was stably integrated into Saccharomyces cerevisiae and afforded forskolin titers of 40 mg/L.DOI:http://dx.doi.org/10.7554/eLife.23001.001 Twit Text FOAVip Total biosynthesis of the cyclic AMP booster forskolin from Coleus forskohlii ????eLife http://www.kidney.de/pget.php?&tw=1&pmid=28290983 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28290983 #FOAvip English Wikipedia <ref>{{Cite pmid|28290983}}</ref> Total biosynthesis of the cyclic AMP booster forskolin from Coleus forskohlii #MMPMID28290983 Pateraki I; Andersen-Ranberg J; Jensen NB; Wubshet SG; Heskes AM; Forman V; Hallström B; Hamberger B; Motawia MS; Olsen CE; Staerk D; Hansen J; Møller BL; Hamberger B eLife 2017[]; 6 (ä): ä PMID28290983show ga Forskolin is a unique structurally complex labdane-type diterpenoid used in the treatment of glaucoma and heart failure based on its activity as a cyclic AMP booster. Commercial production of forskolin relies exclusively on extraction from its only known natural source, the plant Coleus forskohlii, in which forskolin accumulates in the root cork. Here, we report the discovery of five cytochrome P450s and two acetyltransferases which catalyze a cascade of reactions converting the forskolin precursor 13R-manoyl oxide into forskolin and a diverse array of additional labdane-type diterpenoids. A minimal set of three P450s in combination with a single acetyl transferase was identified that catalyzes the conversion of 13R-manoyl oxide into forskolin as demonstrated by transient expression in Nicotiana benthamiana. The entire pathway for forskolin production from glucose encompassing expression of nine genes was stably integrated into Saccharomyces cerevisiae and afforded forskolin titers of 40 mg/L.DOI:http://dx.doi.org/10.7554/eLife.23001.001 äTotal biosynthesis of the cyclic AMP booster forskolin from Coleus for(epmc).pdf DeepDyve Pubget Overpricing