Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1093/nar/gkw907 http://scihub22266oqcxt.onion/10.1093/nar/gkw907 C5388431!5388431!28180329     free     free     free       Nucleic+Acids+Res 2017 ; 45 (3): e15 Nephropedia Template TP {{tp|p=28180329|t=2017. Analysis of translation using polysome profiling |pdf=|usr=}}{{28180329}} gab.com Text Analysis of translation using polysome profiling JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28180329 #FOAvip During the past decade, there has been growing interest in the role of translational regulation of gene expression in many organisms. Polysome profiling has been developed to infer the translational status of a specific mRNA species or to analyze the translatome, i.e. the subset of mRNAs actively translated in a cell. Polysome profiling is especially suitable for emergent model organisms for which genomic data are limited. In this paper, we describe an optimized protocol for the purification of sea urchin polysomes and highlight the critical steps involved in polysome purification. We applied this protocol to obtain experimental results on translational regulation of mRNAs following fertilization. Our protocol should prove useful for integrating the study of the role of translational regulation in gene regulatory networks in any biological model. In addition, we demonstrate how to carry out high-throughput processing of polysome gradient fractions, for the simultaneous screening of multiple biological conditions and large-scale preparation of samples for next-generation sequencing. Twit Text FOAVip Analysis of translation using polysome profiling ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28180329 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28180329 #FOAvip English Wikipedia <ref>{{Cite pmid|28180329}}</ref> Analysis of translation using polysome profiling #MMPMID28180329 Chassé H; Boulben S; Costache V; Cormier P; Morales J Nucleic Acids Res 2017[Feb]; 45 (3): e15 PMID28180329show ga During the past decade, there has been growing interest in the role of translational regulation of gene expression in many organisms. Polysome profiling has been developed to infer the translational status of a specific mRNA species or to analyze the translatome, i.e. the subset of mRNAs actively translated in a cell. Polysome profiling is especially suitable for emergent model organisms for which genomic data are limited. In this paper, we describe an optimized protocol for the purification of sea urchin polysomes and highlight the critical steps involved in polysome purification. We applied this protocol to obtain experimental results on translational regulation of mRNAs following fertilization. Our protocol should prove useful for integrating the study of the role of translational regulation in gene regulatory networks in any biological model. In addition, we demonstrate how to carry out high-throughput processing of polysome gradient fractions, for the simultaneous screening of multiple biological conditions and large-scale preparation of samples for next-generation sequencing. äAnalysis of translation using polysome profiling (epmc).pdf DeepDyve Pubget Overpricing