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10.1093/nar/gkw1250 http://scihub22266oqcxt.onion/10.1093/nar/gkw1250 C5388411!5388411!28180312     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2017 ; 45 (3): 1392-403 Nephropedia Template TP {{tp|p=28180312|t=2017. Restriction glycosylases: involvement of endonuclease activities in the restriction process |pdf=|usr=}}{{28180312}} gab.com Text Restriction glycosylases: involvement of endonuclease activities in the restriction process JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28180312 #FOAvip All restriction enzymes examined are phosphodiesterases generating 3?-OH and 5?-P ends, but one restriction enzyme (restriction glycosylase) excises unmethylated bases from its recognition sequence. Whether its restriction activity involves endonucleolytic cleavage remains unclear. One report on this enzyme, R.PabI from a hyperthermophile, ascribed the breakage to high temperature while another showed its weak AP lyase activity generates atypical ends. Here, we addressed this issue in mesophiles. We purified R.PabI homologs from Campylobacter coli (R.CcoLI) and Helicobacter pylori (R.HpyAXII) and demonstrated their DNA cleavage, DNA glycosylase and AP lyase activities in vitro at 37°C. The AP lyase activity is more coupled with glycosylase activity in R.CcoLI than in R.PabI. R.CcoLI/R.PabI expression caused restriction of incoming bacteriophage/plasmid DNA and endogenous chromosomal DNA within Escherichia coli at 37°C. The R.PabI-mediated restriction was promoted by AP endonuclease action in vivo or in vitro. These results reveal the role of endonucleolytic DNA cleavage in restriction and yet point to diversity among the endonucleases. The cleaved ends are difficult to repair in vivo, which may indicate their biological significance. These results support generalization of the concept of restriction?modification system to the concept of self-recognizing epigenetic system, which combines any epigenetic labeling and any DNA damaging. Twit Text FOAVip Restriction glycosylases: involvement of endonuclease activities in the restriction process ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28180312 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28180312 #FOAvip English Wikipedia <ref>{{Cite pmid|28180312}}</ref> Restriction glycosylases: involvement of endonuclease activities in the restriction process #MMPMID28180312 Zhang Y; Matsuzaka T; Yano H; Furuta Y; Nakano T; Ishikawa K; Fukuyo M; Takahashi N; Suzuki Y; Sugano S; Ide H; Kobayashi I Nucleic Acids Res 2017[Feb]; 45 (3): 1392-403 PMID28180312show ga All restriction enzymes examined are phosphodiesterases generating 3?-OH and 5?-P ends, but one restriction enzyme (restriction glycosylase) excises unmethylated bases from its recognition sequence. Whether its restriction activity involves endonucleolytic cleavage remains unclear. One report on this enzyme, R.PabI from a hyperthermophile, ascribed the breakage to high temperature while another showed its weak AP lyase activity generates atypical ends. Here, we addressed this issue in mesophiles. We purified R.PabI homologs from Campylobacter coli (R.CcoLI) and Helicobacter pylori (R.HpyAXII) and demonstrated their DNA cleavage, DNA glycosylase and AP lyase activities in vitro at 37°C. The AP lyase activity is more coupled with glycosylase activity in R.CcoLI than in R.PabI. R.CcoLI/R.PabI expression caused restriction of incoming bacteriophage/plasmid DNA and endogenous chromosomal DNA within Escherichia coli at 37°C. The R.PabI-mediated restriction was promoted by AP endonuclease action in vivo or in vitro. These results reveal the role of endonucleolytic DNA cleavage in restriction and yet point to diversity among the endonucleases. The cleaved ends are difficult to repair in vivo, which may indicate their biological significance. These results support generalization of the concept of restriction?modification system to the concept of self-recognizing epigenetic system, which combines any epigenetic labeling and any DNA damaging. äRestriction glycosylases: involvement of endonuclease activities in th(epmc).pdf DeepDyve Pubget Overpricing