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A Novel Cervical Spinal Cord Window Preparation Allows for Two-Photon Imaging of
T-Cell Interactions with the Cervical Spinal Cord Microvasculature during
Experimental Autoimmune Encephalomyelitis
#MMPMID28443093
Haghayegh Jahromi N
; Tardent H
; Enzmann G
; Deutsch U
; Kawakami N
; Bittner S
; Vestweber D
; Zipp F
; Stein JV
; Engelhardt B
Front Immunol
2017[]; 8
(?): 406
PMID28443093
show ga
T-cell migration across the blood-brain barrier (BBB) is a crucial step in the
pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model
of multiple sclerosis (MS). Two-photon intravital microscopy (2P-IVM) has been
established as a powerful tool to study cell-cell interactions in inflammatory
EAE lesions in living animals. In EAE, central nervous system inflammation is
strongly pronounced in the spinal cord, an organ in which 2P-IVM imaging is
technically very challenging and has been limited to the lumbar spinal cord.
Here, we describe a novel spinal cord window preparation allowing to use 2P-IVM
to image immune cell interactions with the cervical spinal cord microvascular
endothelium during EAE. We describe differences in the angioarchitecture of the
cervical spinal cord versus the lumbar spinal cord, which will entail different
hemodynamic parameters in these different vascular beds. Using T cells as an
example, we demonstrate the suitability of this novel methodology in imaging the
post-arrest multistep T-cell extravasation across the cervical spinal cord
microvessels. The novel methodology includes an outlook to the analysis of the
cellular pathway of T-cell diapedesis across the BBB by establishing
visualization of endothelial junctions in this vascular bed.
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