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2017 ; 7
(4
): 1323-1337
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Planar Cell Polarity Effector Fritz Interacts with Dishevelled and Has Multiple
Functions in Regulating PCP
#MMPMID28258110
Wang Y
; Naturale VF
; Adler PN
G3 (Bethesda)
2017[Apr]; 7
(4
): 1323-1337
PMID28258110
show ga
The Planar cell Polarity Effector (PPE) genes inturned, fuzzy, and fritz are
downstream components in the frizzled/starry night signaling pathway, and their
function is instructed by upstream Planar Cell Polarity (PCP) core genes such as
frizzled and dishevelled PPE proteins accumulate asymmetrically in wing cells and
function in a protein complex mediated by direct interactions between In and Frtz
and In and Fy. How the PCP proteins instruct the accumulation of PPE protein is
unknown. We found a likely direct interaction between Dishevelled and Fritz and
Dishevelled and Fuzzy that could play a role in this. We previously found that
mild overexpression of frtz rescued a weak in allele. To determine if this was
due to extra Frtz stabilizing mutant In or due to Frtz being able to bypass the
need for In we generate a precise deletion of the inturned gene (in(PD) ). We
found that mild overexpression of Fritz partially rescued in(PD) , indicating
that fritz has In independent activity in PCP. Previous studies of PPE proteins
used fixed tissues, and did not provide any insights into the dynamic properties
of PPE proteins. We used CRISPR/Cas9 genome editing technology to edit the fritz
gene to add a green fluorescent protein tag. fritz(m)(NeonGreen) provides
complete rescue activity and works well for in vivo imaging. Our data showed that
Fritz is very dynamic in epidermal cells and preferentially distributed to
discrete membrane subdomains ("puncta"). Surprisingly, we found it in stripes in
developing bristles.
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