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10.1016/j.celrep.2016.06.011

http://scihub22266oqcxt.onion/10.1016/j.celrep.2016.06.011
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C5384574!5384574!27346360
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suck abstract from ncbi


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pmid27346360      Cell+Rep 2016 ; 16 (2): 571-82
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  • A fluorescent reporter mouse for inflammasome assembly demonstrates an important role for cell bound and free ASC specks during in vivo infection #MMPMID27346360
  • Tzeng TCJ; Schattgen S; Monks B; Wang D; Cerny A; Latz E; Fitzgerald K; Golenbock DT
  • Cell Rep 2016[Jul]; 16 (2): 571-82 PMID27346360show ga
  • Inflammasome activation is associated with numerous diseases. However, in vivo detection of the activated inflammasome complex has been limited by a dearth of tools. We developed transgenic mice that ectopically express the fluorescent adaptor protein, ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain), and characterized the formation of assembled inflammasome complexes (?specks?) in primary cells and tissues. In addition to hematopoietic cells, we found that a stromal population in the lung tissues forms specks during the early phase of influenza infection whereas myeloid cells showed speck formation after two days. In a peritonitis and Group B streptococcus infection models, a higher percentage of neutrophils formed specks at early phases of infection, while dendritic cells formed specks at later time points. Furthermore, speck-forming cells underwent pyroptosis, and extensive release of specks to the extracellular milieu in vivo. These data underscore the importance of free specks during inflammatory processes in vivo.
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