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10.1038/ncomms14738 http://scihub22266oqcxt.onion/10.1038/ncomms14738 C5384221!5384221!28382928     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2017 ; 8 (ä): ä Nephropedia Template TP {{tp|p=28382928|t=2017. DNA origami-based shape IDs for single-molecule nanomechanical genotyping |pdf=|usr=}}{{28382928}} gab.com Text DNA origami-based shape IDs for single-molecule nanomechanical genotyping JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28382928 #FOAvip Variations on DNA sequences profoundly affect how we develop diseases and respond to pathogens and drugs. Atomic force microscopy (AFM) provides a nanomechanical imaging approach for genetic analysis with nanometre resolution. However, unlike fluorescence imaging that has wavelength-specific fluorophores, the lack of shape-specific labels largely hampers widespread applications of AFM imaging. Here we report the development of a set of differentially shaped, highly hybridizable self-assembled DNA origami nanostructures serving as shape IDs for magnified nanomechanical imaging of single-nucleotide polymorphisms. Using these origami shape IDs, we directly genotype single molecules of human genomic DNA with an ultrahigh resolution of ?10?nm and the multiplexing ability. Further, we determine three types of disease-associated, long-range haplotypes in samples from the Han Chinese population. Single-molecule analysis allows robust haplotyping even for samples with low labelling efficiency. We expect this generic shape ID-based nanomechanical approach to hold great potential in genetic analysis at the single-molecule level. Twit Text FOAVip DNA origami-based shape IDs for single-molecule nanomechanical genotyping ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28382928 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28382928 #FOAvip English Wikipedia <ref>{{Cite pmid|28382928}}</ref> DNA origami-based shape IDs for single-molecule nanomechanical genotyping #MMPMID28382928 Zhang H; Chao J; Pan D; Liu H; Qiang Y; Liu K; Cui C; Chen J; Huang Q; Hu J; Wang L; Huang W; Shi Y; Fan C Nat Commun 2017[]; 8 (ä): ä PMID28382928show ga Variations on DNA sequences profoundly affect how we develop diseases and respond to pathogens and drugs. Atomic force microscopy (AFM) provides a nanomechanical imaging approach for genetic analysis with nanometre resolution. However, unlike fluorescence imaging that has wavelength-specific fluorophores, the lack of shape-specific labels largely hampers widespread applications of AFM imaging. Here we report the development of a set of differentially shaped, highly hybridizable self-assembled DNA origami nanostructures serving as shape IDs for magnified nanomechanical imaging of single-nucleotide polymorphisms. Using these origami shape IDs, we directly genotype single molecules of human genomic DNA with an ultrahigh resolution of ?10?nm and the multiplexing ability. Further, we determine three types of disease-associated, long-range haplotypes in samples from the Han Chinese population. Single-molecule analysis allows robust haplotyping even for samples with low labelling efficiency. We expect this generic shape ID-based nanomechanical approach to hold great potential in genetic analysis at the single-molecule level. äDNA origami-based shape IDs for single-molecule nanomechanical genotyp(epmc).pdf DeepDyve Pubget Overpricing