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10.1038/srep46170 http://scihub22266oqcxt.onion/10.1038/srep46170 C5383999!5383999!28387307     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2017 ; 7 (ä): ä Nephropedia Template TP {{tp|p=28387307|t=2017. Stomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1) |pdf=|usr=}}{{28387307}} gab.com Text Stomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1) JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28387307 #FOAvip Anion Exchanger 1 (AE1) and stomatin are integral proteins of the red blood cell (RBC) membrane. Erythroid and kidney AE1 play a major role in HCO3? and Cl? exchange. Stomatins down-regulate the activity of many channels and transporters. Biochemical studies suggested an interaction of erythroid AE1 with stomatin. Moreover, we previously reported normal AE1 expression level in stomatin-deficient RBCs. Here, the ability of stomatin to modulate AE1-dependent Cl?/HCO3? exchange was evaluated using stopped-flow methods. In HEK293 cells expressing recombinant AE1 and stomatin, the permeabilities associated with AE1 activity were 30% higher in cells overexpressing stomatin, compared to cells with only endogenous stomatin expression. Ghosts from stomatin-deficient RBCs and controls were resealed in the presence of pH- or chloride-sensitive fluorescent probes and submitted to inward HCO3? and outward Cl? gradients. From alkalinization rate constants, we deduced a 47% decreased permeability to HCO3? for stomatin-deficient patients. Similarly, kinetics of Cl? efflux, followed by the probe dequenching, revealed a significant 42% decrease in patients. In situ Proximity Ligation Assays confirmed an interaction of AE1 with stomatin, in both HEK recombinant cells and RBCs. Here we show that stomatin modulates the transport activity of AE1 through a direct protein-protein interaction. Twit Text FOAVip Stomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1) ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28387307 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28387307 #FOAvip English Wikipedia <ref>{{Cite pmid|28387307}}</ref> Stomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1) #MMPMID28387307 Genetet S; Desrames A; Chouali Y; Ripoche P; Lopez C; Mouro-Chanteloup I Sci Rep 2017[]; 7 (ä): ä PMID28387307show ga Anion Exchanger 1 (AE1) and stomatin are integral proteins of the red blood cell (RBC) membrane. Erythroid and kidney AE1 play a major role in HCO3? and Cl? exchange. Stomatins down-regulate the activity of many channels and transporters. Biochemical studies suggested an interaction of erythroid AE1 with stomatin. Moreover, we previously reported normal AE1 expression level in stomatin-deficient RBCs. Here, the ability of stomatin to modulate AE1-dependent Cl?/HCO3? exchange was evaluated using stopped-flow methods. In HEK293 cells expressing recombinant AE1 and stomatin, the permeabilities associated with AE1 activity were 30% higher in cells overexpressing stomatin, compared to cells with only endogenous stomatin expression. Ghosts from stomatin-deficient RBCs and controls were resealed in the presence of pH- or chloride-sensitive fluorescent probes and submitted to inward HCO3? and outward Cl? gradients. From alkalinization rate constants, we deduced a 47% decreased permeability to HCO3? for stomatin-deficient patients. Similarly, kinetics of Cl? efflux, followed by the probe dequenching, revealed a significant 42% decrease in patients. In situ Proximity Ligation Assays confirmed an interaction of AE1 with stomatin, in both HEK recombinant cells and RBCs. Here we show that stomatin modulates the transport activity of AE1 through a direct protein-protein interaction. äStomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1)(epmc).pdf DeepDyve Pubget Overpricing