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10.1186/s12866-017-0994-6 http://scihub22266oqcxt.onion/10.1186/s12866-017-0994-6 C5381085!5381085 !28376716     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28376716 &cmd=llinks): Failed to open stream: HTTP request failed! 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In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) |pdf=|usr=}}{{28376716 }} gab.com Text In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) JO: BMC Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=28376716 #FOAvip BACKGROUND: Staphylococcus aureus more than any other human pathogen is a better model for the study of the adaptive evolution of bacterial resistance to antibiotics, as it has demonstrated a remarkable ability in its response to new antibiotics. This study was designed to investigate the in vitro transfer of mecA gene from methicillin resistant S. aureus to methicillin susceptible S. aureus. RESULT: The recipient transconjugants were resistant to erythromycin, cefpodoxime and were mecA positive. PCR amplification of mecA after mix culture plating on Luria Bertani agar containing 100 ?g/mL showed that 75% of the donor and 58.3% of the recipient transconjugants were mecA positive. Additionally, 61.5% of both the donor cells and recipient transconjugants were mecA positive, while 46.2% and 41.75% of both donor and recipient transconjugants were mecA positive on LB agar containing 50 ?g/mL and 30 ?g/mL respectively. CONCLUSION: In this study, the direction of transfer of phenotypic resistance as well as mecA was observed to have occurred from the donor to the recipient strains. This study affirmed the importance of horizontal transfer events in the dissemination of antibiotics resistance among different strains of MRSA. Twit Text FOAVip In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) ????BMC Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=28376716 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28376716 #FOAvip English Wikipedia <ref>{{Cite pmid|28376716 }}</ref> In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) #MMPMID28376716 Bitrus AA ; Zunita Z ; Bejo SK ; Othman S ; Nadzir NA BMC Microbiol 2017[Apr]; 17 (1 ): 83 PMID28376716 show ga BACKGROUND: Staphylococcus aureus more than any other human pathogen is a better model for the study of the adaptive evolution of bacterial resistance to antibiotics, as it has demonstrated a remarkable ability in its response to new antibiotics. This study was designed to investigate the in vitro transfer of mecA gene from methicillin resistant S. aureus to methicillin susceptible S. aureus. RESULT: The recipient transconjugants were resistant to erythromycin, cefpodoxime and were mecA positive. PCR amplification of mecA after mix culture plating on Luria Bertani agar containing 100 ?g/mL showed that 75% of the donor and 58.3% of the recipient transconjugants were mecA positive. Additionally, 61.5% of both the donor cells and recipient transconjugants were mecA positive, while 46.2% and 41.75% of both donor and recipient transconjugants were mecA positive on LB agar containing 50 ?g/mL and 30 ?g/mL respectively. CONCLUSION: In this study, the direction of transfer of phenotypic resistance as well as mecA was observed to have occurred from the donor to the recipient strains. This study affirmed the importance of horizontal transfer events in the dissemination of antibiotics resistance among different strains of MRSA. |Bacterial Proteins/*genetics [MESH] |Base Sequence [MESH] |DNA, Bacterial [MESH] |Gene Transfer, Horizontal/genetics [MESH] |Genes, Bacterial [MESH] |In Vitro Techniques [MESH] |Methicillin Resistance/*genetics [MESH] |Methicillin-Resistant Staphylococcus aureus/*drug effects/*genetics [MESH] |Methicillin/*pharmacology [MESH] |Microbial Sensitivity Tests [MESH] |Penicillin-Binding Proteins/*genetics [MESH] |Polymerase Chain Reaction [MESH] |Staphylococcus aureus/*genetics/pathogenicity [MESH]In vitro transfer of methicillin resistance determinants mecA from met(epmc).pdf DeepDyve Pubget Overpricing