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10.1038/nprot.2015.119 http://scihub22266oqcxt.onion/10.1038/nprot.2015.119 C5380003!5380003!26492138     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Protoc 2015 ; 10 (11): 1802-19 Nephropedia Template TP {{tp|p=26492138|t=2015. Imaging the beating heart in the mouse using intravital microscopy techniques |pdf=|usr=}}{{26492138}} gab.com Text Imaging the beating heart in the mouse using intravital microscopy techniques JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=26492138 #FOAvip Real-time microscopic imaging of moving organs at single-cell resolution represents a major challenge in studying complex biology in living systems. Motion of the tissue from the cardiac and respiratory cycles severely limits intravital microscopy by compromising ultimate spatial and temporal imaging resolution. However, significant recent advances have enabled single-cell resolution imaging to be achieved in vivo. In this protocol, we describe experimental procedures for intravital microscopy based on a combination of thoracic surgery, tissue stabilizers and acquisition gating methods, which enable imaging at the single-cell level in the beating heart in the mouse. Setup of the model is typically completed in 1 h, which allows 2 h or more of continuous cardiac imaging. This protocol can be readily adapted for the imaging of other moving organs, and it will therefore broadly facilitate in vivo high-resolution microscopy studies. Twit Text FOAVip Imaging the beating heart in the mouse using intravital microscopy techniques ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=26492138 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26492138 #FOAvip English Wikipedia <ref>{{Cite pmid|26492138}}</ref> Imaging the beating heart in the mouse using intravital microscopy techniques #MMPMID26492138 Vinegoni C; Aguirre AD; Lee S; Weissleder R Nat Protoc 2015[Nov]; 10 (11): 1802-19 PMID26492138show ga Real-time microscopic imaging of moving organs at single-cell resolution represents a major challenge in studying complex biology in living systems. Motion of the tissue from the cardiac and respiratory cycles severely limits intravital microscopy by compromising ultimate spatial and temporal imaging resolution. However, significant recent advances have enabled single-cell resolution imaging to be achieved in vivo. In this protocol, we describe experimental procedures for intravital microscopy based on a combination of thoracic surgery, tissue stabilizers and acquisition gating methods, which enable imaging at the single-cell level in the beating heart in the mouse. Setup of the model is typically completed in 1 h, which allows 2 h or more of continuous cardiac imaging. This protocol can be readily adapted for the imaging of other moving organs, and it will therefore broadly facilitate in vivo high-resolution microscopy studies. äImaging the beating heart in the mouse using intravital microscopy tec(epmc).pdf DeepDyve Pubget Overpricing