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2017 ; 13
(4
): 1329-1336
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MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3
in renal cancer cells
#MMPMID28413473
Qiu M
; Liang Z
; Chen L
; Tan G
; Liu L
; Wang K
; Chen H
; Liu J
Exp Ther Med
2017[Apr]; 13
(4
): 1329-1336
PMID28413473
show ga
The aim of the present study was to evaluate the functions of miR-200c in the
regulation of tumor growth and metastasis in renal cancer cells, and to
investigate the underlying mechanisms. In this study, miR-200c was up- and
downregulated in two renal cancer cell lines, namely ACHN and A498, and the
proliferation, colony formation, migration and invasion of the cells were
measured. The expression levels of various mRNAs and proteins were then analyzed
using reverse transcription-quantitative polymerase chain reaction and western
blotting, respectively. It was found that miR-200c suppressed proliferation,
migration and invasion of the renal cancer cells and, conversely, the inhibition
of endogenous miR-200c resulted in increased cell proliferation and metastasis.
Furthermore, a luciferase reporter assay revealed that miR-200c directly targeted
the 3' untranslated regions of the oncogenes B-cell-specific Moloney murine
leukemia virus insertion site 1 (Bmi-1) and E2F transcription factor 3 (E2F3)
mRNAs, reduced the expression of Bmi-1 and E2F3 and regulated the expression of
downstream genes, including E-cadherin, N-cadherin, vimentin, p14 and p16. These
results indicate a tumor suppressor role for miR-200c in renal cancer cells via
the direct targeting of Bmi-1 and E2F3.
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