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10.1016/B978-0-12-420138-5.00008-2 http://scihub22266oqcxt.onion/10.1016/B978-0-12-420138-5.00008-2 C5376065!5376065!24974026     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Cell+Biol 2014 ; 123 (ä): 135-51 Nephropedia Template TP {{tp|p=24974026|t=2014. Assessing and benchmarking multiphoton microscopes for biologists |pdf=|usr=}}{{24974026}} gab.com Text Assessing and benchmarking multiphoton microscopes for biologists JO: Methods Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=24974026 #FOAvip Multiphoton microscopy has become staple tool for tracking cells within tissues and organs due to superior depth of penetration, low excitation volumes, and reduced phototoxicity. Many factors, ranging from laser pulse width to relay optics to detectors and electronics, contribute to the overall ability of these microscopes to excite and detect fluorescence deep within tissues. However, we have found that there are few standard ways already described in the literature to distinguish between microscopes or to benchmark existing microscopes to measure the overall quality and efficiency of these instruments. Here, we discuss some simple parameters and methods that can either be used within a multiphoton facility or by a prospective purchaser to benchmark performance. This can both assist in identifying decay in microscope performance and in choosing features of a scope that are suited to experimental needs. Twit Text FOAVip Assessing and benchmarking multiphoton microscopes for biologists ????Methods Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=24974026 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24974026 #FOAvip English Wikipedia <ref>{{Cite pmid|24974026}}</ref> Assessing and benchmarking multiphoton microscopes for biologists #MMPMID24974026 Corbin K; Pinkard H; Peck S; Beemiller P; Krummel MF Methods Cell Biol 2014[]; 123 (ä): 135-51 PMID24974026show ga Multiphoton microscopy has become staple tool for tracking cells within tissues and organs due to superior depth of penetration, low excitation volumes, and reduced phototoxicity. Many factors, ranging from laser pulse width to relay optics to detectors and electronics, contribute to the overall ability of these microscopes to excite and detect fluorescence deep within tissues. However, we have found that there are few standard ways already described in the literature to distinguish between microscopes or to benchmark existing microscopes to measure the overall quality and efficiency of these instruments. Here, we discuss some simple parameters and methods that can either be used within a multiphoton facility or by a prospective purchaser to benchmark performance. This can both assist in identifying decay in microscope performance and in choosing features of a scope that are suited to experimental needs. äAssessing and benchmarking multiphoton microscopes for biologists (epmc).pdf DeepDyve Pubget Overpricing