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10.1242/dev.145367 http://scihub22266oqcxt.onion/10.1242/dev.145367 C5374353!5374353!28174243     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Development 2017 ; 144 (5): 928-34 Nephropedia Template TP {{tp|p=28174243|t=2017. Efficient TALEN-mediated gene targeting of chicken primordial germ cells |pdf=|usr=}}{{28174243}} gab.com Text Efficient TALEN-mediated gene targeting of chicken primordial germ cells JO: Development http://www.kidney.de/pget.php?&tw=1&pmid=28174243 #FOAvip In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 (vasa) locus in chicken primordial germ cells (PGCs). Vasa is a key germ cell determinant in many animal species and is posited to control avian germ cell formation. We show that TALENs mediate homology-directed repair of the DDX4 locus on the Z sex chromosome at high (8.1%) efficiencies. Large genetic deletions of 30?kb encompassing the entire DDX4 locus were also created using a single TALEN pair. The targeted PGCs were germline competent and were used to produce DDX4 null offspring. In DDX4 knockout chickens, PGCs are initially formed but are lost during meiosis in the developing ovary, leading to adult female sterility. TALEN-mediated gene targeting in avian PGCs is therefore an efficient process. Twit Text FOAVip Efficient TALEN-mediated gene targeting of chicken primordial germ cells ????Development http://www.kidney.de/pget.php?&tw=1&pmid=28174243 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28174243 #FOAvip English Wikipedia <ref>{{Cite pmid|28174243}}</ref> Efficient TALEN-mediated gene targeting of chicken primordial germ cells #MMPMID28174243 Taylor L; Carlson DF; Nandi S; Sherman A; Fahrenkrug SC; McGrew MJ Development 2017[Mar]; 144 (5): 928-34 PMID28174243show ga In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 (vasa) locus in chicken primordial germ cells (PGCs). Vasa is a key germ cell determinant in many animal species and is posited to control avian germ cell formation. We show that TALENs mediate homology-directed repair of the DDX4 locus on the Z sex chromosome at high (8.1%) efficiencies. Large genetic deletions of 30?kb encompassing the entire DDX4 locus were also created using a single TALEN pair. The targeted PGCs were germline competent and were used to produce DDX4 null offspring. In DDX4 knockout chickens, PGCs are initially formed but are lost during meiosis in the developing ovary, leading to adult female sterility. TALEN-mediated gene targeting in avian PGCs is therefore an efficient process. äEfficient TALEN-mediated gene targeting of chicken primordial germ cel(epmc).pdf DeepDyve Pubget Overpricing