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10.1038/srep45623 http://scihub22266oqcxt.onion/10.1038/srep45623 C5372366!5372366 !28358123     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28358123 &cmd=llinks): Failed to open stream: HTTP request failed! 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Molecular Therapy for Degenerative Disc Disease: Clues from Secretome Analysis of the Notochordal Cell-Rich Nucleus Pulposus |pdf=|usr=}}{{28358123 }} gab.com Text Molecular Therapy for Degenerative Disc Disease: Clues from Secretome Analysis of the Notochordal Cell-Rich Nucleus Pulposus JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28358123 #FOAvip Degenerative disc disease (DDD) is associated with spinal pain often leading to long-term disability. However, the non-chondrodystrophic canine intervertebral disc is protected from the development of DDD, ostensibly due to its retention of notochordal cells (NC) in the nucleus pulposus (NP). In this study, we hypothesized that secretome analysis of the NC-rich NP will lead to the identification of key proteins that delay the onset of DDD. Using mass-spectrometry, we identified 303 proteins including components of TGF?- and Wnt-signaling, anti-angiogeneic factors and proteins that inhibit axonal ingrowth in the bioactive fractions of serum free, notochordal cell derived conditioned medium (NCCM). Ingenuity Pathway Analysis revealed TGF?1 and CTGF as major hubs in protein interaction networks. In vitro treatment with TGF?1 and CTGF promoted the synthesis of healthy extra-cellular matrix proteins, increased cell proliferation and reduced cell death in human degenerative disc NP cells. A single intra-discal injection of recombinant TGF?1 and CTGF proteins in a pre-clinical rat-tail disc injury model restored the NC and stem cell rich NP. In conclusion, we demonstrate the potential of TGF?1 and CTGF to mitigate the progression of disc degeneration and the potential use of these molecules in a molecular therapy to treat the degenerative disc. Twit Text FOAVip Molecular Therapy for Degenerative Disc Disease: Clues from Secretome Analysis of the Notochordal Cell-Rich Nucleus Pulposus ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28358123 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28358123 #FOAvip English Wikipedia <ref>{{Cite pmid|28358123 }}</ref> Molecular Therapy for Degenerative Disc Disease: Clues from Secretome Analysis of the Notochordal Cell-Rich Nucleus Pulposus #MMPMID28358123 Matta A ; Karim MZ ; Isenman DE ; Erwin WM Sci Rep 2017[Mar]; 7 (ä): 45623 PMID28358123 show ga Degenerative disc disease (DDD) is associated with spinal pain often leading to long-term disability. However, the non-chondrodystrophic canine intervertebral disc is protected from the development of DDD, ostensibly due to its retention of notochordal cells (NC) in the nucleus pulposus (NP). In this study, we hypothesized that secretome analysis of the NC-rich NP will lead to the identification of key proteins that delay the onset of DDD. Using mass-spectrometry, we identified 303 proteins including components of TGF?- and Wnt-signaling, anti-angiogeneic factors and proteins that inhibit axonal ingrowth in the bioactive fractions of serum free, notochordal cell derived conditioned medium (NCCM). Ingenuity Pathway Analysis revealed TGF?1 and CTGF as major hubs in protein interaction networks. In vitro treatment with TGF?1 and CTGF promoted the synthesis of healthy extra-cellular matrix proteins, increased cell proliferation and reduced cell death in human degenerative disc NP cells. A single intra-discal injection of recombinant TGF?1 and CTGF proteins in a pre-clinical rat-tail disc injury model restored the NC and stem cell rich NP. In conclusion, we demonstrate the potential of TGF?1 and CTGF to mitigate the progression of disc degeneration and the potential use of these molecules in a molecular therapy to treat the degenerative disc. |Animals [MESH] |CCN Intercellular Signaling Proteins/metabolism [MESH] |Cell Survival [MESH] |Cells, Cultured [MESH] |Connective Tissue Growth Factor/metabolism [MESH] |Culture Media, Conditioned [MESH] |Disease Models, Animal [MESH] |Female [MESH] |Humans [MESH] |Intervertebral Disc Degeneration/*metabolism/pathology/*therapy [MESH] |Mass Spectrometry [MESH] |Notochord/*metabolism [MESH] |Nucleus Pulposus/*metabolism [MESH] |Protein Interaction Maps [MESH] |Rats, Wistar [MESH] |Repressor Proteins/metabolism [MESH] |Signal Transduction [MESH]Molecular Therapy for Degenerative Disc Disease: Clues from Secretome (epmc).pdf DeepDyve Pubget Overpricing