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10.18632/oncotarget.15218 http://scihub22266oqcxt.onion/10.18632/oncotarget.15218 C5370017!5370017!28199983     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Oncotarget 2017 ; 8 (10): 17002-11 Nephropedia Template TP {{tp|p=28199983|t=2017. A versatile system for rapid multiplex genome-edited CAR T cell generation |pdf=|usr=}}{{28199983}} gab.com Text A versatile system for rapid multiplex genome-edited CAR T cell generation JO: Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=28199983 #FOAvip The therapeutic potential of CRISPR system has already been demonstrated in many instances and begun to overlap with the rapidly expanding field of cancer immunotherapy, especially on the production of genetically modified T cell receptor or chimeric antigen receptor (CAR) T cells. Efficient genomic disruption of multiple gene loci to generate universal donor cells, as well as potent effector T cells resistant to multiple inhibitory pathways such as PD-1 and CTLA4 is an attractive strategy for cell therapy. In this study, we accomplished rapid and efficient multiplex genomic editing, and re-directing T cells with antigen specific CAR via a one-shot CRISPR protocol by incorporation of multiple gRNAs in a CAR lentiviral vector. High efficient double knockout of endogenous TCR and HLA class I could be easily achieved to generate allogeneic universal CAR T cells. We also generated Fas-resistant universal CAR T cells by triple gene disruption. Simultaneous gene editing of four gene loci using the one-shot CRISPR protocol to generate allogeneic universal T cells deficient of both PD1 and CTLA-4 was also attempted. Twit Text FOAVip A versatile system for rapid multiplex genome-edited CAR T cell generation ????Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=28199983 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28199983 #FOAvip English Wikipedia <ref>{{Cite pmid|28199983}}</ref> A versatile system for rapid multiplex genome-edited CAR T cell generation #MMPMID28199983 Ren J; Zhang X; Liu X; Fang C; Jiang S; June CH; Zhao Y Oncotarget 2017[Mar]; 8 (10): 17002-11 PMID28199983show ga The therapeutic potential of CRISPR system has already been demonstrated in many instances and begun to overlap with the rapidly expanding field of cancer immunotherapy, especially on the production of genetically modified T cell receptor or chimeric antigen receptor (CAR) T cells. Efficient genomic disruption of multiple gene loci to generate universal donor cells, as well as potent effector T cells resistant to multiple inhibitory pathways such as PD-1 and CTLA4 is an attractive strategy for cell therapy. In this study, we accomplished rapid and efficient multiplex genomic editing, and re-directing T cells with antigen specific CAR via a one-shot CRISPR protocol by incorporation of multiple gRNAs in a CAR lentiviral vector. High efficient double knockout of endogenous TCR and HLA class I could be easily achieved to generate allogeneic universal CAR T cells. We also generated Fas-resistant universal CAR T cells by triple gene disruption. Simultaneous gene editing of four gene loci using the one-shot CRISPR protocol to generate allogeneic universal T cells deficient of both PD1 and CTLA-4 was also attempted. äA versatile system for rapid multiplex genome-edited CAR T cell genera(epmc).pdf DeepDyve Pubget Overpricing