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2017 ; 36
(4
): 677-680
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Dalbavancin reduces biofilms of methicillin-resistant Staphylococcus aureus
(MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE)
#MMPMID27896496
Knafl D
; Tobudic S
; Cheng SC
; Bellamy DR
; Thalhammer F
Eur J Clin Microbiol Infect Dis
2017[Apr]; 36
(4
): 677-680
PMID27896496
show ga
Activity of dalbavancin against methicillin-resistant Staphylococcus aureus
(MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE) in biofilm was
investigated and the microbicidal biofilm concentrations (MBC) were determined.
Biofilms obtained from ten MRSA and ten MRSE bloodstream isolates, collected from
patients in the General Hospital of Vienna between 2012 and 2015, were incubated
with dalbavancin in trypticase soy broth (TSB) in serial dilution from
0.0625 mg/l to 256 mg/l using a microtiter plate biofilm model. The plates were
incubated for 24 h at 37 ° C and 50% humidity. Biofilms were fixed with 2.5%
glutaraldehyde and stained with crystal violet. Subsequently the optical density
(OD(620)) was used to measure the MBC, defined as the concentration of
dalbavancin leading to a 50% reduction of biofilm. MBC for MRSA was 1 mg/l-4 mg/l
(minimal inhibitory concentrations (MIC) 0.0312 mg/l-0.064 mg/l). MBC for MRSE
was 2 mg/l-16 mg/l (MIC 0.023 mg/l-0.0625 mg/l). Dalbavancin successfully reduced
MRSA and MRSE in biofilms, and therefore provides a promising option for the
treatment of biofilm-associated infections.