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10.1038/ncomms14843 http://scihub22266oqcxt.onion/10.1038/ncomms14843 C5364421!5364421!28327660     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2017 ; 8 (ä): ä Nephropedia Template TP {{tp|p=28327660|t=2017. Quantitative volumetric Raman imaging of three dimensional cell cultures |pdf=|usr=}}{{28327660}} gab.com Text Quantitative volumetric Raman imaging of three dimensional cell cultures JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28327660 #FOAvip The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell?material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy. Twit Text FOAVip Quantitative volumetric Raman imaging of three dimensional cell cultures ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28327660 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28327660 #FOAvip English Wikipedia <ref>{{Cite pmid|28327660}}</ref> Quantitative volumetric Raman imaging of three dimensional cell cultures #MMPMID28327660 Kallepitis C; Bergholt MS; Mazo MM; Leonardo V; Skaalure SC; Maynard SA; Stevens MM Nat Commun 2017[]; 8 (ä): ä PMID28327660show ga The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell?material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy. äQuantitative volumetric Raman imaging of three dimensional cell cultur(epmc).pdf DeepDyve Pubget Overpricing