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2016 ; 7
(47
): 76726-76734
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The first investigation of Wilms tumour atomic structure-nitrogen and carbon
isotopic composition as a novel biomarker for the most individual approach in
cancer disease
#MMPMID27732932
Taran K
; Fr?czek T
; Sikora-Szubert A
; Sitkiewicz A
; M?ynarski W
; Kobos J
; Paneth P
Oncotarget
2016[Nov]; 7
(47
): 76726-76734
PMID27732932
show ga
The paper describes a novel approach to investigating Wilms' tumour
(nephroblastoma) biology at the atomic level. Isotope Ratio Mass Spectrometry
(IRMS) was used to directly assess the isotope ratios of nitrogen and carbon in
84 Wilms' tumour tissue samples from 28 cases representing the histological
spectrum of nephroblastoma. Marked differences in nitrogen and carbon isotope
ratios were found between nephroblastoma histological types and along the course
of cancer disease, with a breakout in isotope ratio of the examined elements in
tumour tissue found between stages 2 and 3. Different isotopic compositions with
regard to nitrogen and carbon content were observed in blastemal Wilms' tumour,
with and without focal anaplasia, and in poorly- and well-differentiated
epithelial nephroblastoma. This first assessment of nitrogen and carbon isotope
ratio reveals the previously unknown part of Wilms' tumour biology and represents
a potential novel biomarker, allowing for a highly individual approach to
treating cancer. Furthermore, this method of estimating isotopic composition
appears to be the most sensitive tool yet for cancer tissue evaluation, and a
valuable complement to established cancer study methods with prospective clinical
impact.