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10.1016/j.omtn.2017.01.006 http://scihub22266oqcxt.onion/10.1016/j.omtn.2017.01.006 C5363512!5363512!28325296     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Ther+Nucleic+Acids 2017 ; 6 (ä): 290-301 Nephropedia Template TP {{tp|p=28325296|t=2017. An Atelocollagen Coating for Efficient Local Gene Silencing by Using Small Interfering RNA |pdf=|usr=}}{{28325296}} gab.com Text An Atelocollagen Coating for Efficient Local Gene Silencing by Using Small Interfering RNA JO: Mol Ther Nucleic Acids http://www.kidney.de/pget.php?&tw=1&pmid=28325296 #FOAvip In the last decades, many efforts have been made to counteract adverse effects after stenting atherosclerotic coronary arteries. A breakthrough in better vascular wall regeneration was noted in the new era of drug-eluting stents. A novel personalized approach is the development of gene-eluting stents promising an alteration in gene expression involved in regeneration. We investigated a coating system consisting of the polymer atelocollagen (ATCOL) and a specific small interfering RNA (siRNA) for intercellular adhesion molecule-1 (ICAM-1) found on the surface of defective endothelial cells (ECs). We demonstrated very high cell viability, in which EA.hy926 grew on 0.008% or 0.032% ATCOL layers. Additionally, hemocompatibility assays proved the biocompatibility of this coating. The highest transfection efficiency with EA.hy926 was achieved with 5 ?g siRNA immobilized in ATCOL after 2 days. The release of fluorescent-labeled siRNA was about 9 days. Long-term knockdown of ICAM-1 was analyzed by flow cytometry, revealing that the coating with 0.008% ATCOL and 5 ?g siICAM-1 provoked gene silencing up to 8 days. 5?-RNA ligase-mediated rapid amplification of cDNA ends PCR (RLM-RACE-PCR) demonstrated the specificity of our established ATCOL gene-silencing coating, meaning that our coating is well suited for further investigations in in vivo studies. Herein, we would like to demonstrate that our ATCOL is well-suited for better artery wall regeneration after stent implantation. Twit Text FOAVip An Atelocollagen Coating for Efficient Local Gene Silencing by Using Small Interfering RNA ????Mol Ther Nucleic Acids http://www.kidney.de/pget.php?&tw=1&pmid=28325296 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28325296 #FOAvip English Wikipedia <ref>{{Cite pmid|28325296}}</ref> An Atelocollagen Coating for Efficient Local Gene Silencing by Using Small Interfering RNA #MMPMID28325296 Koenig O; Nothdurft D; Perle N; Neumann B; Behring A; Degenkolbe I; Walker T; Schlensak C; Wendel HP; Nolte A Mol Ther Nucleic Acids 2017[Mar]; 6 (ä): 290-301 PMID28325296show ga In the last decades, many efforts have been made to counteract adverse effects after stenting atherosclerotic coronary arteries. A breakthrough in better vascular wall regeneration was noted in the new era of drug-eluting stents. A novel personalized approach is the development of gene-eluting stents promising an alteration in gene expression involved in regeneration. We investigated a coating system consisting of the polymer atelocollagen (ATCOL) and a specific small interfering RNA (siRNA) for intercellular adhesion molecule-1 (ICAM-1) found on the surface of defective endothelial cells (ECs). We demonstrated very high cell viability, in which EA.hy926 grew on 0.008% or 0.032% ATCOL layers. Additionally, hemocompatibility assays proved the biocompatibility of this coating. The highest transfection efficiency with EA.hy926 was achieved with 5 ?g siRNA immobilized in ATCOL after 2 days. The release of fluorescent-labeled siRNA was about 9 days. Long-term knockdown of ICAM-1 was analyzed by flow cytometry, revealing that the coating with 0.008% ATCOL and 5 ?g siICAM-1 provoked gene silencing up to 8 days. 5?-RNA ligase-mediated rapid amplification of cDNA ends PCR (RLM-RACE-PCR) demonstrated the specificity of our established ATCOL gene-silencing coating, meaning that our coating is well suited for further investigations in in vivo studies. Herein, we would like to demonstrate that our ATCOL is well-suited for better artery wall regeneration after stent implantation. äAn Atelocollagen Coating for Efficient Local Gene Silencing by Using S(epmc).pdf DeepDyve Pubget Overpricing