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10.1371/journal.ppat.1006270 http://scihub22266oqcxt.onion/10.1371/journal.ppat.1006270 C5362246!5362246!28282445     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Pathog 2017 ; 13 (3): ä Nephropedia Template TP {{tp|p=28282445|t=2017. Endothelial cell tropism is a determinant of H5N1 pathogenesis in mammalian species |pdf=|usr=}}{{28282445}} gab.com Text Endothelial cell tropism is a determinant of H5N1 pathogenesis in mammalian species JO: PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=28282445 #FOAvip The cellular and molecular mechanisms underpinning the unusually high virulence of highly pathogenic avian influenza H5N1 viruses in mammalian species remains unknown. Here, we investigated if the cell tropism of H5N1 virus is a determinant of enhanced virulence in mammalian species. We engineered H5N1 viruses with restricted cell tropism through the exploitation of cell type-specific microRNA expression by incorporating microRNA target sites into the viral genome. Restriction of H5N1 replication in endothelial cells via miR-126 ameliorated disease symptoms, prevented systemic viral spread and limited mortality, despite showing similar levels of peak viral replication in the lungs as compared to control virus-infected mice. Similarly, restriction of H5N1 replication in endothelial cells resulted in ameliorated disease symptoms and decreased viral spread in ferrets. Our studies demonstrate that H5N1 infection of endothelial cells results in excessive production of cytokines and reduces endothelial barrier integrity in the lungs, which culminates in vascular leakage and viral pneumonia. Importantly, our studies suggest a need for a combinational therapy that targets viral components, suppresses host immune responses, and improves endothelial barrier integrity for the treatment of highly pathogenic H5N1 virus infections. Twit Text FOAVip Endothelial cell tropism is a determinant of H5N1 pathogenesis in mammalian species ????PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=28282445 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28282445 #FOAvip English Wikipedia <ref>{{Cite pmid|28282445}}</ref> Endothelial cell tropism is a determinant of H5N1 pathogenesis in mammalian species #MMPMID28282445 Tundup S; Kandasamy M; Perez JT; Mena N; Steel J; Nagy T; Albrecht RA; Manicassamy B PLoS Pathog 2017[Mar]; 13 (3): ä PMID28282445show ga The cellular and molecular mechanisms underpinning the unusually high virulence of highly pathogenic avian influenza H5N1 viruses in mammalian species remains unknown. Here, we investigated if the cell tropism of H5N1 virus is a determinant of enhanced virulence in mammalian species. We engineered H5N1 viruses with restricted cell tropism through the exploitation of cell type-specific microRNA expression by incorporating microRNA target sites into the viral genome. Restriction of H5N1 replication in endothelial cells via miR-126 ameliorated disease symptoms, prevented systemic viral spread and limited mortality, despite showing similar levels of peak viral replication in the lungs as compared to control virus-infected mice. Similarly, restriction of H5N1 replication in endothelial cells resulted in ameliorated disease symptoms and decreased viral spread in ferrets. Our studies demonstrate that H5N1 infection of endothelial cells results in excessive production of cytokines and reduces endothelial barrier integrity in the lungs, which culminates in vascular leakage and viral pneumonia. Importantly, our studies suggest a need for a combinational therapy that targets viral components, suppresses host immune responses, and improves endothelial barrier integrity for the treatment of highly pathogenic H5N1 virus infections. äEndothelial cell tropism is a determinant of H5N1 pathogenesis in mamm(epmc).pdf DeepDyve Pubget Overpricing