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10.1242/jcs.195420 http://scihub22266oqcxt.onion/10.1242/jcs.195420 C5358338!5358338!28137756     free     free     free       J+Cell+Sci 2017 ; 130 (6): 1147-57 Nephropedia Template TP {{tp|p=28137756|t=2017. Endocytic turnover of Rab8 controls cell polarization |pdf=|usr=}}{{28137756}} gab.com Text Endocytic turnover of Rab8 controls cell polarization JO: J Cell Sci http://www.kidney.de/pget.php?&tw=1&pmid=28137756 #FOAvip Adaptation of cell shape and polarization through the formation and retraction of cellular protrusions requires balancing of endocytosis and exocytosis combined with fine-tuning of the local activity of small GTPases like Rab8. Here, we show that endocytic turnover of the plasma membrane at protrusions is directly coupled to surface removal and inactivation of Rab8. Removal is induced by reduced membrane tension and mediated by the GTPase regulator associated with focal adhesion kinase-1 (GRAF1, also known as ARHGAP26), a regulator of clathrin-independent endocytosis. GRAF1-depleted cells were deficient in multi-directional spreading and displayed elevated levels of GTP-loaded Rab8, which was accumulated at the tips of static protrusions. Furthermore, GRAF1 depletion impaired lumen formation and spindle orientation in a 3D cell culture system, indicating that GRAF1 activity regulates polarity establishment. Our data suggest that GRAF1-mediated removal of Rab8 from the cell surface restricts its activity during protrusion formation, thereby facilitating dynamic adjustment of the polarity axis. Twit Text FOAVip Endocytic turnover of Rab8 controls cell polarization ????J Cell Sci http://www.kidney.de/pget.php?&tw=1&pmid=28137756 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28137756 #FOAvip English Wikipedia <ref>{{Cite pmid|28137756}}</ref> Endocytic turnover of Rab8 controls cell polarization #MMPMID28137756 Vidal-Quadras M; Holst MR; Francis MK; Larsson E; Hachimi M; Yau WL; Peränen J; Martín-Belmonte F; Lundmark R J Cell Sci 2017[Mar]; 130 (6): 1147-57 PMID28137756show ga Adaptation of cell shape and polarization through the formation and retraction of cellular protrusions requires balancing of endocytosis and exocytosis combined with fine-tuning of the local activity of small GTPases like Rab8. Here, we show that endocytic turnover of the plasma membrane at protrusions is directly coupled to surface removal and inactivation of Rab8. Removal is induced by reduced membrane tension and mediated by the GTPase regulator associated with focal adhesion kinase-1 (GRAF1, also known as ARHGAP26), a regulator of clathrin-independent endocytosis. GRAF1-depleted cells were deficient in multi-directional spreading and displayed elevated levels of GTP-loaded Rab8, which was accumulated at the tips of static protrusions. Furthermore, GRAF1 depletion impaired lumen formation and spindle orientation in a 3D cell culture system, indicating that GRAF1 activity regulates polarity establishment. Our data suggest that GRAF1-mediated removal of Rab8 from the cell surface restricts its activity during protrusion formation, thereby facilitating dynamic adjustment of the polarity axis. äEndocytic turnover of Rab8 controls cell polarization (epmc).pdf DeepDyve Pubget Overpricing