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10.1242/dev.142950 http://scihub22266oqcxt.onion/10.1242/dev.142950 C5358112!5358112!28219945     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Development 2017 ; 144 (6): 1113-7 Nephropedia Template TP {{tp|p=28219945|t=2017. Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging |pdf=|usr=}}{{28219945}} gab.com Text Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging JO: Development http://www.kidney.de/pget.php?&tw=1&pmid=28219945 #FOAvip Tissue, organ and organoid cultures provide suitable models for developmental studies, but our understanding of how the organs are assembled at the single-cell level still remains unclear. We describe here a novel fixed z-direction (FiZD) culture setup that permits high-resolution confocal imaging of organoids and embryonic tissues. In a FiZD culture a permeable membrane compresses the tissues onto a glass coverslip and the spacers adjust the thickness, enabling the tissue to grow for up to 12?days. Thus, the kidney rudiment and the organoids can adjust to the limited z-directional space and yet advance the process of kidney morphogenesis, enabling long-term time-lapse and high-resolution confocal imaging. As the data quality achieved was sufficient for computer-assisted cell segmentation and analysis, the method can be used for studying morphogenesis ex vivo at the level of the single constituent cells of a complex mammalian organogenesis model system. Twit Text FOAVip Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging ????Development http://www.kidney.de/pget.php?&tw=1&pmid=28219945 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28219945 #FOAvip English Wikipedia <ref>{{Cite pmid|28219945}}</ref> Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging #MMPMID28219945 Saarela U; Akram SU; Desgrange A; Rak-Raszewska A; Shan J; Cereghini S; Ronkainen VP; Heikkilä J; Skovorodkin I; Vainio SJ Development 2017[Mar]; 144 (6): 1113-7 PMID28219945show ga Tissue, organ and organoid cultures provide suitable models for developmental studies, but our understanding of how the organs are assembled at the single-cell level still remains unclear. We describe here a novel fixed z-direction (FiZD) culture setup that permits high-resolution confocal imaging of organoids and embryonic tissues. In a FiZD culture a permeable membrane compresses the tissues onto a glass coverslip and the spacers adjust the thickness, enabling the tissue to grow for up to 12?days. Thus, the kidney rudiment and the organoids can adjust to the limited z-directional space and yet advance the process of kidney morphogenesis, enabling long-term time-lapse and high-resolution confocal imaging. As the data quality achieved was sufficient for computer-assisted cell segmentation and analysis, the method can be used for studying morphogenesis ex vivo at the level of the single constituent cells of a complex mammalian organogenesis model system. äNovel fixed z-direction (FiZD) kidney primordia and an organoid cultur(epmc).pdf DeepDyve Pubget Overpricing