Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.kint.2016.10.015 http://scihub22266oqcxt.onion/10.1016/j.kint.2016.10.015 C5357449!5357449 !27998643     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27998643 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Kidney+Int 2017 ; 91 (4 ): 896-913 Nephropedia Template TP {{tp|p=27998643 |t=2017. Activated ERK1/2 increases CD44 in glomerular parietal epithelial cells leading to matrix expansion |pdf=|usr=}}{{27998643 }} gab.com Text Activated ERK1/2 increases CD44 in glomerular parietal epithelial cells leading to matrix expansion JO: Kidney Int http://www.kidney.de/pget.php?&tw=1&pmid=27998643 #FOAvip The glycoprotein CD44 is barely detected in normal mouse and human glomeruli, but is increased in glomerular parietal epithelial cells following podocyte injury in focal segmental glomerulosclerosis (FSGS). To determine the biological role and regulation of CD44 in these cells, we employed an in vivo and in vitro approach. Experimental FSGS was induced in CD44 knockout and wild-type mice with a cytotoxic podocyte antibody. Albuminuria, focal and global glomerulosclerosis (periodic acid-Schiff stain), and collagen IV staining were lower in CD44 knockout compared with wild-type mice with FSGS. Parietal epithelial cells had lower migration from Bowman's capsule to the glomerular tuft in CD44 knockout mice with disease compared with wild type mice. In cultured murine parietal epithelial cells, overexpressing CD44 with a retroviral vector encoding CD44 was accompanied by significantly increased collagen IV expression and parietal epithelial cell migration. Because our results showed de novo co-staining for activated ERK1/2 (pERK) in parietal epithelial cells in experimental FSGS, and also in biopsies from patients with FSGS, two in vitro strategies were employed to prove that pERK regulated CD44 levels. First, mouse parietal epithelial cells were infected with a retroviral vector for the upstream kinase MEK-DD to increase pERK, which was accompanied by increased CD44 levels. Second, in CD44-overexpressing parietal epithelial cells, decreasing pERK with U0126 was accompanied by reduced CD44. Finally, parietal epithelial cell migration was higher in cells with increased and reduced in cells with decreased pERK. Thus, pERK is a regulator of CD44 expression, and increased CD44 expression leads to a pro-sclerotic and migratory parietal epithelial cell phenotype. Twit Text FOAVip Activated ERK1/2 increases CD44 in glomerular parietal epithelial cells leading to matrix expansion ????Kidney Int http://www.kidney.de/pget.php?&tw=1&pmid=27998643 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27998643 #FOAvip English Wikipedia <ref>{{Cite pmid|27998643 }}</ref> Activated ERK1/2 increases CD44 in glomerular parietal epithelial cells leading to matrix expansion #MMPMID27998643 Roeder SS ; Barnes TJ ; Lee JS ; Kato I ; Eng DG ; Kaverina NV ; Sunseri MW ; Daniel C ; Amann K ; Pippin JW ; Shankland SJ Kidney Int 2017[Apr]; 91 (4 ): 896-913 PMID27998643 show ga The glycoprotein CD44 is barely detected in normal mouse and human glomeruli, but is increased in glomerular parietal epithelial cells following podocyte injury in focal segmental glomerulosclerosis (FSGS). To determine the biological role and regulation of CD44 in these cells, we employed an in vivo and in vitro approach. Experimental FSGS was induced in CD44 knockout and wild-type mice with a cytotoxic podocyte antibody. Albuminuria, focal and global glomerulosclerosis (periodic acid-Schiff stain), and collagen IV staining were lower in CD44 knockout compared with wild-type mice with FSGS. Parietal epithelial cells had lower migration from Bowman's capsule to the glomerular tuft in CD44 knockout mice with disease compared with wild type mice. In cultured murine parietal epithelial cells, overexpressing CD44 with a retroviral vector encoding CD44 was accompanied by significantly increased collagen IV expression and parietal epithelial cell migration. Because our results showed de novo co-staining for activated ERK1/2 (pERK) in parietal epithelial cells in experimental FSGS, and also in biopsies from patients with FSGS, two in vitro strategies were employed to prove that pERK regulated CD44 levels. First, mouse parietal epithelial cells were infected with a retroviral vector for the upstream kinase MEK-DD to increase pERK, which was accompanied by increased CD44 levels. Second, in CD44-overexpressing parietal epithelial cells, decreasing pERK with U0126 was accompanied by reduced CD44. Finally, parietal epithelial cell migration was higher in cells with increased and reduced in cells with decreased pERK. Thus, pERK is a regulator of CD44 expression, and increased CD44 expression leads to a pro-sclerotic and migratory parietal epithelial cell phenotype. |Albuminuria/enzymology/genetics/prevention & control [MESH] |Animals [MESH] |Cell Movement [MESH] |Cells, Cultured [MESH] |Collagen Type IV/metabolism [MESH] |Disease Models, Animal [MESH] |Enzyme Activation [MESH] |Extracellular Matrix/drug effects/*enzymology/pathology [MESH] |Genetic Predisposition to Disease [MESH] |Glomerulosclerosis, Focal Segmental/*enzymology/genetics/pathology [MESH] |Humans [MESH] |Hyaluronan Receptors/genetics/*metabolism [MESH] |Kidney Glomerulus/drug effects/*enzymology/pathology [MESH] |Male [MESH] |Mice, Knockout [MESH] |Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/*metabolism [MESH] |Mitogen-Activated Protein Kinase 3/antagonists & inhibitors/*metabolism [MESH] |Phenotype [MESH] |Phosphorylation [MESH] |Podocytes/drug effects/*enzymology/pathology [MESH] |Protein Kinase Inhibitors/pharmacology [MESH] |Signal Transduction [MESH] |Time Factors [MESH]Activated ERK1/2 increases CD44 in glomerular parietal epithelial cell(epmc).pdf DeepDyve Pubget Overpricing