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2017 ; 18
(1
): 176
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Using simulated fluorescence cell micrographs for the evaluation of cell image
segmentation algorithms
#MMPMID28315633
Wiesmann V
; Bergler M
; Palmisano R
; Prinzen M
; Franz D
; Wittenberg T
BMC Bioinformatics
2017[Mar]; 18
(1
): 176
PMID28315633
show ga
BACKGROUND: Manual assessment and evaluation of fluorescent micrograph cell
experiments is time-consuming and tedious. Automated segmentation pipelines can
ensure efficient and reproducible evaluation and analysis with constant high
quality for all images of an experiment. Such cell segmentation approaches are
usually validated and rated in comparison to manually annotated micrographs.
Nevertheless, manual annotations are prone to errors and display inter- and
intra-observer variability which influence the validation results of automated
cell segmentation pipelines. RESULTS: We present a new approach to simulate
fluorescent cell micrographs that provides an objective ground truth for the
validation of cell segmentation methods. The cell simulation was evaluated
twofold: (1) An expert observer study shows that the proposed approach generates
realistic fluorescent cell micrograph simulations. (2) An automated segmentation
pipeline on the simulated fluorescent cell micrographs reproduces segmentation
performances of that pipeline on real fluorescent cell micrographs. CONCLUSION:
The proposed simulation approach produces realistic fluorescent cell micrographs
with corresponding ground truth. The simulated data is suited to evaluate image
segmentation pipelines more efficiently and reproducibly than it is possible on
manually annotated real micrographs.