Transcription factor NF?YA promotes a malignant phenotype by upregulating fatty
acid synthase expression
#MMPMID27840951
Guo J
; Kong LM
; Peng AF
; Long XH
; Zhou Y
; Shu Y
Mol Med Rep
2016[Dec]; 14
(6
): 5007-5014
PMID27840951
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Recent studies have revealed that increased expression of the alpha subunit of
nuclear transcription factor Y (NF?YA) is associated with the malignant phenotype
of various tumors. However, whether elevated expression of NF?YA promotes a
malignant phenotype in osteosarcoma (OS), and the molecular mechanisms underlying
this predicted effect is currently unknown. In the present study, small hairpin
RNA (shRNA)?mediated knockdown of endogenous NF?YA significantly inhibited the
migration and invasion capabilities of OS cells in vitro, whereas ectopic
expression of NF?YA increased the migration and invasion capabilities of these
cells. In addition, the induction of upregulated NF?YA expression on the
malignant phenotype of OS cells was attenuated by silencing fatty acid synthase
(FASN) expression. Furthermore, the expression level of FASN was increased by
upregulating NF?YA, while decreased FASN expression was observed following NF?YA
silencing in OS cells. The results of the present study suggest that NF?YA may
promote a malignant phenotype in OS cells, in part, by activating the FASN
signaling pathway, which may represent a promising target for the management of
OS.
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