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10.3892/mmr.2016.5899 http://scihub22266oqcxt.onion/10.3892/mmr.2016.5899 C5355694!5355694 !27840965     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27840965 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Med+Rep 2016 ; 14 (6 ): 4991-4998 Nephropedia Template TP {{tp|p=27840965 |t=2016. Breast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics |pdf=|usr=}}{{27840965 }} gab.com Text Breast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics JO: Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=27840965 #FOAvip Identification and isolation of breast cancer stem cells (CSCs) based on CD44/CD24 expression and/or enzymatic activity of aldehyde dehydrogenase 1 (ALDH1). However, the differences among the CD44+/CD24?/low cells, ALDH1+ cells and the overlap between the sub?populations have not been frequently investigated. Thus, it is imperative to improve the understanding of breast CSC with different stem markers. CD44+/CD24?/low, ALDH1+ and ALDH1+CD44+/CD24?/low cell populations were isolated from fresh breast cancer tissues and analyzed by flow cytometry and immunofluorescence. Mammosphere formation, cell proliferation assay and Transwell experiments, were used to analyze self?renewal, proliferation and invasion, respectively, for each sub?population. Finally, in vivo experimentation in mice was performed to evaluate the tumorigenic abilities of the sub?populations. The sub?populations of CD44+/CD24?/low, ALDH1+ and ALDH1+CD44+/CD24?/low in human breast cancer cells, represented the 7.2, 4.6 and 1.5% of the total tumor cell population, respectively. ALDH1+CD44+/CD24?/low cells had the strongest ability of self?renewal, invasion, proliferation and tumorigenicity compared with the other sub?populations (P<0.05). In conclusion, different phenotypes of CD44+/CD24?/low, ALDH1+ and ALDH1+CD44+/CD24?/low were isolated and demonstrated that breast CSCs are heterogeneous, and they exhibit distinct biological characteristics. As ALDH1+CD44+/CD24?/low cells demonstrated the strongest stem?like properties, it may be a useful specific stem cell marker. The utilization of more reliable biomarkers to distinguish the breast CSC pool will be important for the development of specific target therapies for breast cancer. Twit Text FOAVip Breast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics ????Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=27840965 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27840965 #FOAvip English Wikipedia <ref>{{Cite pmid|27840965 }}</ref> Breast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics #MMPMID27840965 Shao J ; Fan W ; Ma B ; Wu Y Mol Med Rep 2016[Dec]; 14 (6 ): 4991-4998 PMID27840965 show ga Identification and isolation of breast cancer stem cells (CSCs) based on CD44/CD24 expression and/or enzymatic activity of aldehyde dehydrogenase 1 (ALDH1). However, the differences among the CD44+/CD24?/low cells, ALDH1+ cells and the overlap between the sub?populations have not been frequently investigated. Thus, it is imperative to improve the understanding of breast CSC with different stem markers. CD44+/CD24?/low, ALDH1+ and ALDH1+CD44+/CD24?/low cell populations were isolated from fresh breast cancer tissues and analyzed by flow cytometry and immunofluorescence. Mammosphere formation, cell proliferation assay and Transwell experiments, were used to analyze self?renewal, proliferation and invasion, respectively, for each sub?population. Finally, in vivo experimentation in mice was performed to evaluate the tumorigenic abilities of the sub?populations. The sub?populations of CD44+/CD24?/low, ALDH1+ and ALDH1+CD44+/CD24?/low in human breast cancer cells, represented the 7.2, 4.6 and 1.5% of the total tumor cell population, respectively. ALDH1+CD44+/CD24?/low cells had the strongest ability of self?renewal, invasion, proliferation and tumorigenicity compared with the other sub?populations (P<0.05). In conclusion, different phenotypes of CD44+/CD24?/low, ALDH1+ and ALDH1+CD44+/CD24?/low were isolated and demonstrated that breast CSCs are heterogeneous, and they exhibit distinct biological characteristics. As ALDH1+CD44+/CD24?/low cells demonstrated the strongest stem?like properties, it may be a useful specific stem cell marker. The utilization of more reliable biomarkers to distinguish the breast CSC pool will be important for the development of specific target therapies for breast cancer. |*Biomarkers [MESH] |Adult [MESH] |Animals [MESH] |Breast Neoplasms/genetics/*metabolism/*pathology [MESH] |Cell Line, Tumor [MESH] |Cell Movement/genetics [MESH] |Cell Proliferation [MESH] |Disease Models, Animal [MESH] |Female [MESH] |Fluorescent Antibody Technique [MESH] |Gene Expression Regulation, Neoplastic [MESH] |Heterografts [MESH] |Humans [MESH] |Immunophenotyping [MESH] |Mice [MESH] |Neoplastic Stem Cells/*metabolism [MESH] |Phenotype [MESH] |Spheroids, Cellular [MESH]Breast cancer stem cells expressing different stem cell markers exhibi(epmc).pdf DeepDyve Pubget Overpricing