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10.1074/jbc.M116.742346 http://scihub22266oqcxt.onion/10.1074/jbc.M116.742346 C5354483!5354483 !28115519     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28115519 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Biol+Chem 2017 ; 292 (10 ): 4266-4279 Nephropedia Template TP {{tp|p=28115519 |t=2017. Comparative Characterization of Phosphatidic Acid Sensors and Their Localization during Frustrated Phagocytosis |pdf=|usr=}}{{28115519 }} gab.com Text Comparative Characterization of Phosphatidic Acid Sensors and Their Localization during Frustrated Phagocytosis JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28115519 #FOAvip Phosphatidic acid (PA) is the simplest phospholipid naturally existing in living organisms, but it constitutes only a minor fraction of total cell lipids. PA has attracted considerable attention because it is a phospholipid precursor, a lipid second messenger, and a modulator of membrane shape, and it has thus been proposed to play key cellular functions. The dynamics of PA in cells and in subcellular compartments, however, remains an open question. The recent generation of fluorescent probes for PA, by fusing GFP to PA-binding domains, has provided direct evidence for PA dynamics in different intracellular compartments. Here, three PA sensors were characterized in vitro, and their preferences for different PA species in particular lipidic environments were compared. In addition, the localization of PA in macrophages during frustrated phagocytosis was examined using these PA sensors and was combined with a lipidomic analysis of PA in intracellular compartments. The results indicate that the PA sensors display some preferences for specific PA species, depending on the lipid environment, and the localization study in macrophages revealed the complexity of intracellular PA dynamics. Twit Text FOAVip Comparative Characterization of Phosphatidic Acid Sensors and Their Localization during Frustrated Phagocytosis ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28115519 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28115519 #FOAvip English Wikipedia <ref>{{Cite pmid|28115519 }}</ref> Comparative Characterization of Phosphatidic Acid Sensors and Their Localization during Frustrated Phagocytosis #MMPMID28115519 Kassas N ; Tanguy E ; Thahouly T ; Fouillen L ; Heintz D ; Chasserot-Golaz S ; Bader MF ; Grant NJ ; Vitale N J Biol Chem 2017[Mar]; 292 (10 ): 4266-4279 PMID28115519 show ga Phosphatidic acid (PA) is the simplest phospholipid naturally existing in living organisms, but it constitutes only a minor fraction of total cell lipids. PA has attracted considerable attention because it is a phospholipid precursor, a lipid second messenger, and a modulator of membrane shape, and it has thus been proposed to play key cellular functions. The dynamics of PA in cells and in subcellular compartments, however, remains an open question. The recent generation of fluorescent probes for PA, by fusing GFP to PA-binding domains, has provided direct evidence for PA dynamics in different intracellular compartments. Here, three PA sensors were characterized in vitro, and their preferences for different PA species in particular lipidic environments were compared. In addition, the localization of PA in macrophages during frustrated phagocytosis was examined using these PA sensors and was combined with a lipidomic analysis of PA in intracellular compartments. The results indicate that the PA sensors display some preferences for specific PA species, depending on the lipid environment, and the localization study in macrophages revealed the complexity of intracellular PA dynamics. |*Biosensing Techniques [MESH] |Animals [MESH] |Cell Membrane/*metabolism [MESH] |Green Fluorescent Proteins/metabolism [MESH] |Humans [MESH] |Lipids/analysis [MESH] |Macrophages/cytology/*metabolism [MESH] |Phagocytosis/*physiology [MESH] |Phosphatidic Acids/*metabolism [MESH] |Recombinant Fusion Proteins/*metabolism [MESH]Comparative Characterization of Phosphatidic Acid Sensors and Their Lo(epmc).pdf DeepDyve Pubget Overpricing