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10.1074/jbc.M116.767897 http://scihub22266oqcxt.onion/10.1074/jbc.M116.767897 C5354480!5354480 !28154010     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28154010 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Biol+Chem 2017 ; 292 (10 ): 4210-4221 Nephropedia Template TP {{tp|p=28154010 |t=2017. Extracellular Loops Are Essential for the Assembly and Function of Polycystin Receptor-Ion Channel Complexes |pdf=|usr=}}{{28154010 }} gab.com Text Extracellular Loops Are Essential for the Assembly and Function of Polycystin Receptor-Ion Channel Complexes JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28154010 #FOAvip Polycystin complexes, or TRPP-PKD complexes, made of transient receptor potential channel polycystin (TRPP) and polycystic kidney disease (PKD) proteins, play key roles in coupling extracellular stimuli with intracellular Ca(2+) signals. For example, the TRPP2-PKD1 complex has a crucial function in renal physiology, with mutations in either protein causing autosomal dominant polycystic kidney disease. In contrast, the TRPP3-PKD1L3 complex responds to low pH and was proposed to be a sour taste receptor candidate. It has been shown previously that the protein partners interact via association of the C-terminal or transmembrane segments, with consequences for the assembly, surface expression, and function of the polycystin complexes. However, the roles of extracellular components, especially the loops that connect the transmembrane segments, in the assembly and function of the polycystin complex are largely unknown. Here, with an immunoprecipitation method, we found that extracellular loops between the first and second transmembrane segments of TRPP2 and TRPP3 associate with the extracellular loops between the sixth and seventh transmembrane segments of PKD1 and PKD1L3, respectively. Immunofluorescence and electrophysiology data further confirm that the associations between these loops are essential for the trafficking and function of the complexes. Interestingly, most of the extracellular loops are also found to be involved in homomeric assembly. Furthermore, autosomal dominant polycystic kidney disease-associated TRPP2 mutant T448K significantly weakened TRPP2 homomeric assembly but had no obvious effect on TRPP2-PKD1 heteromeric assembly. Our results demonstrate a crucial role of these functionally underexplored extracellular loops in the assembly and function of the polycystin complexes. Twit Text FOAVip Extracellular Loops Are Essential for the Assembly and Function of Polycystin Receptor-Ion Channel Complexes ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28154010 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28154010 #FOAvip English Wikipedia <ref>{{Cite pmid|28154010 }}</ref> Extracellular Loops Are Essential for the Assembly and Function of Polycystin Receptor-Ion Channel Complexes #MMPMID28154010 Salehi-Najafabadi Z ; Li B ; Valentino V ; Ng C ; Martin H ; Yu Y ; Wang Z ; Kashyap P ; Yu Y J Biol Chem 2017[Mar]; 292 (10 ): 4210-4221 PMID28154010 show ga Polycystin complexes, or TRPP-PKD complexes, made of transient receptor potential channel polycystin (TRPP) and polycystic kidney disease (PKD) proteins, play key roles in coupling extracellular stimuli with intracellular Ca(2+) signals. For example, the TRPP2-PKD1 complex has a crucial function in renal physiology, with mutations in either protein causing autosomal dominant polycystic kidney disease. In contrast, the TRPP3-PKD1L3 complex responds to low pH and was proposed to be a sour taste receptor candidate. It has been shown previously that the protein partners interact via association of the C-terminal or transmembrane segments, with consequences for the assembly, surface expression, and function of the polycystin complexes. However, the roles of extracellular components, especially the loops that connect the transmembrane segments, in the assembly and function of the polycystin complex are largely unknown. Here, with an immunoprecipitation method, we found that extracellular loops between the first and second transmembrane segments of TRPP2 and TRPP3 associate with the extracellular loops between the sixth and seventh transmembrane segments of PKD1 and PKD1L3, respectively. Immunofluorescence and electrophysiology data further confirm that the associations between these loops are essential for the trafficking and function of the complexes. Interestingly, most of the extracellular loops are also found to be involved in homomeric assembly. Furthermore, autosomal dominant polycystic kidney disease-associated TRPP2 mutant T448K significantly weakened TRPP2 homomeric assembly but had no obvious effect on TRPP2-PKD1 heteromeric assembly. Our results demonstrate a crucial role of these functionally underexplored extracellular loops in the assembly and function of the polycystin complexes. |Amino Acid Sequence [MESH] |Amino Acid Substitution [MESH] |Calcium Channels [MESH] |Crystallography, X-Ray [MESH] |HEK293 Cells [MESH] |Humans [MESH] |Ion Channels/chemistry/genetics/*metabolism [MESH] |Ion Transport [MESH] |Mutagenesis, Site-Directed [MESH] |Mutation/*genetics [MESH] |Polycystic Kidney, Autosomal Dominant/genetics/metabolism/*pathology [MESH] |Protein Conformation [MESH] |Sequence Homology, Amino Acid [MESH]Extracellular Loops Are Essential for the Assembly and Function of Pol(epmc).pdf DeepDyve Pubget Overpricing