The potential role of Osteopontin in the maintenance of commensal bacteria
homeostasis in the intestine
#MMPMID28296922
Ito K
; Nakajima A
; Fukushima Y
; Suzuki K
; Sakamoto K
; Hamazaki Y
; Ogasawara K
; Minato N
; Hattori M
PLoS One
2017[]; 12
(3
): e0173629
PMID28296922
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Osteopontin (Opn), a multifunctional extracellular matrix protein, is implicated
in the pathogenesis of various inflammatory disorders. Under physiologic
conditions, its expression is restricted to certain tissues including bone and
kidney tubule. However, cellular activation during disease development induces
Opn expression in various immune cells. In this study, using Opn-EGFP knock-in
(KI) mice we found that CD8?+ T cells in the intestinal tissues, including
Peyer's patch, lamina propria and epithelium, express Opn under steady state
conditions. Therefore, we examined the role of Opn-expressing CD8?+ T cells in
intestinal homeostasis. Interestingly, Opn knockout (KO) mice had altered fecal
microflora concordant with a reduction of TCR??+ intraepithelial lymphocytes
(IELs). Consistent with this result, both treatment with anti-Opn blocking
antibody and deficiency of Opn resulted in decreased survival of TCR??+ and
TCR??+ IELs. This data suggests that a possibility that Opn may function as a
survival factor for IELs in the intestinal tissue. Collectively, these data
suggest the possibility that Opn might regulate the homeostasis of intestinal
microflora through maintenance of TCR??+ IELs, possibly by support of IEL
survival.
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