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MicroRNA-320a downregulation mediates human liver cancer cell proliferation
through the Wnt/?-catenin signaling pathway
#MMPMID28356931
Lu C
; Liao Z
; Cai M
; Zhang G
Oncol Lett
2017[Feb]; 13
(2
): 573-578
PMID28356931
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MicroRNAs (miRs) have emerged as key epigenetic regulators involved in cancer
progression. miR-320a has been demonstrated to be a novel tumor suppressive
microRNA in several types of cancers. In the present study, the role of miR-320a
in human hepatocellular carcinoma (HCC) was investigated. The expression levels
of miR-320a and messenger RNA were determined by reverse
transcription-quantitative polymerase chain reaction, while cell cycle and cell
apoptosis were analyzed by flow cytometry. The cell proliferative ability was
determined by Cell Counting Kit-8 assay and colony formation assay. The
downstream target of miR-320a was confirmed by luciferase reporter assay, while
the protein levels were measured by western blotting. The results revealed that
miR-320a was inversely associated with HCC proliferation in HCC cell lines.
Functional studies demonstrated that miR-320a significantly decreased the
capability of cell proliferation and induced G(0)/G(1) growth arrest in vitro. In
addition, ?-catenin was identified as one of the direct targets of miR-320a,
downregulating the expression level of ?-catenin, c-myc, cyclin D1 and
dickkopf-1. In conclusion, miR-320a may act as a tumor-suppressive microRNA
through targeting ?-catenin in HCC.
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